Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene

Autor(es)

de Barros, Patricia Pimentel ; Freire, Fernanda ; Rossoni, Rodnei Dennis ; Junqueira, Juliana Campos ; Cardoso Jorge, Antonio Olavo

• Tipo de documento: Artigo Científico
• Fonte: Folia Microbiologica; v. 62, n. 4, p. 317-323, JUL 2017.
• Citações Web of Science: 2

Resumo

Pathogenicity of Candida albicans is associated with its capacity switch from yeast-like to hyphal growth. The hyphal form is capable to penetrate the epithelial surfaces and to damage the host tissues. Therefore, many investigations have focused on mechanisms that control the morphological transitions of C. albicans. Recently, certain studies have showed that non-albicans Candida species can reduce the capacity of C. albicans to form biofilms and to develop candidiasis in animal models. Then, the objective of this study was to evaluate the effects of Candida krusei and Candida glabrata on the morphogenesis of C. albicans. Firstly, the capacity of reference and clinical strains of C. albicans in forming hyphae was tested in vitro. After that, the expression of HWP1 (hyphal wall protein 1) gene was determined by quantitative real-time PCR (polymerase chain reaction) assay. For both reference and clinical strains, a significant inhibition of the hyphae formation was observed when C. albicans was incubated in the presence of C. krusei or C. glabrata compared to the control group composed only by C. albicans. In addition, the culture mixed of C. albicans-C. krusei or C. albicans-C. glabrata reduced significantly the expression of HWP1 gene of C. albicans in relation to single cultures of this specie. In both filamentation and gene expression assays, C. krusei showed the higher inhibitory activity on the morphogenesis of C. albicans compared to C. glabrata. C. krusei and C. glabrata are capable to reduce the filamentation of C. albicans and consequently decrease the expression of the HWP1 gene. (AU)

• Processo FAPESP: 11/15194-0 - Interações entre diferentes espécies de Candida na candidose experimental em modelos de invertebrados e vertebrados
• Beneficiário: Rodnei Dennis Rossoni
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/15250-0 - AVALIAÇÃO TEMPORAL DA EXPRESSÃO DOS GENES SAP5, SAP9, PLB1, PLB2, LIP9 E LIP10 EM INTERAÇÕES ENTRE ESPÉCIES DE C. albicans, C. tropicalis, C. glabrata E C. krusei
• Beneficiário: Patrícia Pimentel de Barros
• Modalidade de apoio: Bolsas no Brasil - Doutorado