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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Candida krusei and Candida glabrata reduce the filamentation of Candida albicans by downregulating expression of HWP1 gene

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Author(s):
de Barros, Patricia Pimentel ; Freire, Fernanda ; Rossoni, Rodnei Dennis ; Junqueira, Juliana Campos ; Cardoso Jorge, Antonio Olavo
Total Authors: 5
Document type: Journal article
Source: Folia Microbiologica; v. 62, n. 4, p. 317-323, JUL 2017.
Web of Science Citations: 2
Abstract

Pathogenicity of Candida albicans is associated with its capacity switch from yeast-like to hyphal growth. The hyphal form is capable to penetrate the epithelial surfaces and to damage the host tissues. Therefore, many investigations have focused on mechanisms that control the morphological transitions of C. albicans. Recently, certain studies have showed that non-albicans Candida species can reduce the capacity of C. albicans to form biofilms and to develop candidiasis in animal models. Then, the objective of this study was to evaluate the effects of Candida krusei and Candida glabrata on the morphogenesis of C. albicans. Firstly, the capacity of reference and clinical strains of C. albicans in forming hyphae was tested in vitro. After that, the expression of HWP1 (hyphal wall protein 1) gene was determined by quantitative real-time PCR (polymerase chain reaction) assay. For both reference and clinical strains, a significant inhibition of the hyphae formation was observed when C. albicans was incubated in the presence of C. krusei or C. glabrata compared to the control group composed only by C. albicans. In addition, the culture mixed of C. albicans-C. krusei or C. albicans-C. glabrata reduced significantly the expression of HWP1 gene of C. albicans in relation to single cultures of this specie. In both filamentation and gene expression assays, C. krusei showed the higher inhibitory activity on the morphogenesis of C. albicans compared to C. glabrata. C. krusei and C. glabrata are capable to reduce the filamentation of C. albicans and consequently decrease the expression of the HWP1 gene. (AU)

FAPESP's process: 11/15194-0 - Interactions between different Candida species in experimental candidiasis of invertebrates and vertebrates models
Grantee:Rodnei Dennis Rossoni
Support Opportunities: Scholarships in Brazil - Master
FAPESP's process: 12/15250-0 - EVALUATION OF TEMPORAL EXPRESSION OF GENES SAP5, SAP9, PLB1, PLB2, LIP9 LIP10 IN INTERACTIONS BETWEEN SPECIES C. albicans, C. tropicalis, C. glabrata and C. krusei.
Grantee:Patrícia Pimentel de Barros
Support Opportunities: Scholarships in Brazil - Doctorate