Biophysical evaluation of milk-clotting enzymes processed by high pressure

Autor(es)

de Castro Leite Junior, Bruno Ricardo ; Lima Tribst, Alline Artigiani ; Grant, Nicholas J. ; Yada, Rickey Y. ; Cristianini, Marcelo

• Tipo de documento: Artigo Científico
• Fonte: Food Research International; v. 97, p. 116-122, JUL 2017.
• Citações Web of Science: 2

Resumo

High pressure processing (HPP) is able to promote changes in enzymes structure. This study evaluated the effect of HP on the structural changes in milk-clotting enzymes processed under activation conditions for recombinant camel chymosin (212 MPa/5 min/10 degrees C), calf rennet (280 MPa/20 min/25 degrees C), bovine rennet (222 MPa/5 min/23 degrees C), and porcine pepsin (50 MPa/5 min/20 degrees C) and under inactivation conditions for all enzymes (600 MPa/10-min/25 degrees C) including the protease from Rhizomucbr miehei. In general, it was found that the HPP at activation conditions was able to increase the intrinsic fluorescence of samples with high pepsin concentration (porcine pepsin and bovine rennet), increase significantly the surface hydrophobicity and induce changes in secondary structure of all enzymes. Under inactivation conditions, increases in surface hydrophobicity and a reduction of intrinsic fluorescence were observed, suggesting a higher exposure of hydrophobic sites followed by water quenching of Trp residues. Moreover, changes in secondary structure were observed (with minor changes seen in Rhizomucor miehei protease). In conclusion, HPP was able to unfold milk-clotting enzymes even under activation conditions, and the porcine pepsin and bovine rennet were more sensitive to HPP. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

• Processo FAPESP: 12/13509-6 - Aplicação da tecnologia de alta pressão na modificação de enzimas utilizadas na fabricação de queijos
• Beneficiário: Marcelo Cristianini
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/01425-3 - Avaliação biofísica de enzimas coagulantes do leite processadas por tecnologias de alta pressão
• Beneficiário: Bruno Ricardo de Castro Leite Júnior
• Modalidade de apoio: Bolsas no Exterior - Estágio de Pesquisa - Doutorado
• Processo FAPESP: 14/17782-4 - Efeito do processamento de alta pressão isostática em enzimas coagulantes do leite
• Beneficiário: Bruno Ricardo de Castro Leite Júnior
• Modalidade de apoio: Bolsas no Brasil - Doutorado