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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Biophysical evaluation of milk-clotting enzymes processed by high pressure

Full text
Author(s):
de Castro Leite Junior, Bruno Ricardo ; Lima Tribst, Alline Artigiani ; Grant, Nicholas J. ; Yada, Rickey Y. ; Cristianini, Marcelo
Total Authors: 5
Document type: Journal article
Source: Food Research International; v. 97, p. 116-122, JUL 2017.
Web of Science Citations: 2
Abstract

High pressure processing (HPP) is able to promote changes in enzymes structure. This study evaluated the effect of HP on the structural changes in milk-clotting enzymes processed under activation conditions for recombinant camel chymosin (212 MPa/5 min/10 degrees C), calf rennet (280 MPa/20 min/25 degrees C), bovine rennet (222 MPa/5 min/23 degrees C), and porcine pepsin (50 MPa/5 min/20 degrees C) and under inactivation conditions for all enzymes (600 MPa/10-min/25 degrees C) including the protease from Rhizomucbr miehei. In general, it was found that the HPP at activation conditions was able to increase the intrinsic fluorescence of samples with high pepsin concentration (porcine pepsin and bovine rennet), increase significantly the surface hydrophobicity and induce changes in secondary structure of all enzymes. Under inactivation conditions, increases in surface hydrophobicity and a reduction of intrinsic fluorescence were observed, suggesting a higher exposure of hydrophobic sites followed by water quenching of Trp residues. Moreover, changes in secondary structure were observed (with minor changes seen in Rhizomucor miehei protease). In conclusion, HPP was able to unfold milk-clotting enzymes even under activation conditions, and the porcine pepsin and bovine rennet were more sensitive to HPP. (C) 2017 Elsevier Ltd. All rights reserved. (AU)

FAPESP's process: 12/13509-6 - Application of high pressure technology in the modification on enzymes used in cheese manufacturing
Grantee:Marcelo Cristianini
Support Opportunities: Regular Research Grants
FAPESP's process: 16/01425-3 - Biophysical evaluation of milk-clotting enzymes processed by high pressure technologies
Grantee:Bruno Ricardo de Castro Leite Júnior
Support Opportunities: Scholarships abroad - Research Internship - Doctorate
FAPESP's process: 14/17782-4 - Effect of high isostatic pressure processing on milk-clotting enzymes
Grantee:Bruno Ricardo de Castro Leite Júnior
Support Opportunities: Scholarships in Brazil - Doctorate