Prevalence of Extended-Spectrum -Lactamases CTX-M-8 and CTX-M-2-Producing Salmonella Serotypes from Clinical and Nonhuman Isolates in Brazil

Autor(es)

Fernandes, Sueli Aparecida ; Camargo, Carlos Henrique ; Francisco, Gabriela Rodrigues ; Campagnari Bueno, Maria Fernanda ; Garcia, Doroti Oliveira ; Doi, Yohei ; Tiba Casas, Monique Ribeiro

• Tipo de documento: Artigo Científico
• Fonte: MICROBIAL DRUG RESISTANCE; v. 23, n. 5, p. 580-589, JUL 2017.
• Citações Web of Science: 9

Resumo

We characterized extended-spectrum -lactamases (ESBL) enzymes among Salmonella strains isolated in Brazil from 2009 to 2014. Salmonella recovered from both clinical and nonhuman (food, poultry, and environment) sources were subjected to antimicrobial susceptibility testing. -lactamases genes were detected by polymerase chain reaction/sequencing; plasmid profiles and transferability were assessed by S1-pulsed field gel electrophoresis (PFGE). Genetic diversity was evaluated by XbaI-PFGE. Out of 630 Salmonella strains screened, 46 displayed ESBL phenotype, distributed across 11 different serotypes. bla(CTX-M-8) and bla(CTX-M-2) genes were detected at frequencies of 47% and 41%, respectively. bla(SHV-5) and bla(SHV-2) were also detected but in lower frequencies (4%, 2%). bla(TEM-1) gene was detected in 22% of the strains. Most of the ESBL genes were transferable by conjugation, and the respective bla(ESBL) gene was detected in the recipient strain, indicating the location of ESBL determinants on transferable plasmids. XbaI-PFGE revealed genomic diversity of Salmonella Typhimurium bearing bla(CTX-M-2), bla(CTX-M-8), bla(TEM-1), and bla(SHV-2) genes. Salmonella Muenchen (harboring bla(CTX-M-2)) and Salmonella Corvallis (bla(CTX-M-8) and bla(SHV-5)) showed clonal relatedness within respective serotypes. Our findings underscore the occurrence of diverse ESBL genes in several Salmonella serotypes, reinforcing the need for continuous surveillance of resistance genes circulating in human and nonhuman sources. (AU)

• Processo FAPESP: 12/21709-5 - Diversidade genética de Klebsiella pneumoniae produtoras de KPC isoladas de diversos hospitais do Estado de São Paulo
• Beneficiário: Doroti de Oliveira Garcia
• Linha de fomento: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/14907-8 - Caracterização dos genes associados a resistência ao ácido nalidíxico e susceptibilidade reduzida a Ciprofloxacina de cepas de Salmonella spp. isoladas no Estado de São Paulo
• Beneficiário: Monique Ribeiro Tiba Casas
• Linha de fomento: Auxílio à Pesquisa - Regular