Nanocellulose-collagen-apatite composite associated with osteogenic growth peptide for bone regeneration

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Saska, Sybele ; Teixeira, Lucas Novaes ; Spinola de Castro Raucci, Larissa Moreira ; Scarel-Caminaga, Raquel Mantuaneli ; Franchi, Leonardo Pereira ; dos Santos, Raquel Alves ; Santagneli, Silvia Helena ; Capela, Marisa Veiga ; de Oliveira, Paulo Tambasco ; Takahashi, Catarina Satie ; Minarelli Gaspar, Ana Maria ; Messaddeq, Younes ; Lima Ribeiro, Sidney Jose ; Marchetto, Reinaldo

• Tipo de documento: Artigo Científico
• Fonte: International Journal of Biological Macromolecules; v. 103, p. 467-476, OCT 2017.
• Citações Web of Science: 12

Resumo

Despite advances in the field of biomaterials for bone repair/regeneration, some challenges for developing an ideal bone substitute need to be overcome. Herein, this study synthesized and evaluated in vitro a nanocomposite based on bacterial cellulose (BC), collagen (COL), apatite (Ap) and osteogenic growth peptide (OGP) or its C-terminal pentapeptide {[}OGP(10-14)] for bone regeneration purposes. The BC-COL nanocomposites were successfully obtained by carbodiimide-mediated coupling as demonstrated by spectroscopy analysis. SEM, FTIR and P-31 NMR analyses revealed that in situ synthesis to apatite was an effective route for obtaining of bone-like apatite. The OGP-containing (BC-COL)-Ap stimulated the early development of the osteoblastic phenotype. Additionally, the association among collagen, apatite, and OGP peptides enhanced cell growth compared with OGP-containing BC-Ap. Furthermore, none of the nanocomposites showed cytotoxic, genotoxic or mutagenic effects. These promising results suggest that the (BC-COL)-Ap associated with OGP peptides might be considered a potential candidate for bone tissue engineering applications. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 09/09960-1 - Nanocompositos à base de celulose bacteriana para aplicação na regeneração do tecido ósseo.
• Beneficiário: Reinaldo Marchetto
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 08/58776-6 - Materiais compósitos orgânico-inorgânico com fatores de crescimento OGP e OGP (10-14) para regeneração óssea
• Beneficiário: Sybele Saska Specian
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto