Characterization of a novel cystatin type 2 from Rhipicephalus microplus midgut

Autor(es)

Cardoso, Thyago H. S. ; Lu, Stephen ; Gonzalez, Boris R. G. ; Torquato, Ricardo J. S. ; Tanaka, Aparecida S.

• Tipo de documento: Artigo Científico
• Fonte: Biochimie; v. 140, p. 117-121, SEP 2017.
• Citações Web of Science: 1

Resumo

The Rhipicephalus (Boophilus) microplus is an exclusive bovine ectoparasite responsible for the transmission of pathogens that decrease meat, leather and milk productions. Cattle vaccination is an alternative to control tick infestations, but the discovery of potential antigens is still a challenge for researchers. Recently, our group performed a midgut transcriptome of engorged R. microplus tick, and out of 800-ESTs sequences one cystatin-coding sequence was identified and named Rmcystatin-4. In order to understand the physiological role of Rmcystatin-4, the aim of this work was the expression, purification and functional characterization of a novel type 2 cystatin from the tick R. microplus. Rmcystatin-4 gene expression was identified mostly in tick midgut suggesting its possible role in blood digestion control. Our data showed that rRmcystatin-4 was successfully expressed in active form using Pichia pastoris system and the purified inhibitor presented high selectivity to BmCl-1 (Ki = 0.046 nM). Moreover, rRmcystatin-4 was able to impaired BmCl-1 activity towards bovine hemoglobin. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved. (AU)

• Processo FAPESP: 12/03657-8 - Inibidores e proteases de ectoparasitas: relação de estrutura-função e identificação do papel dessas moléculas na interação de vetores de doenças e seus agentes etiológicos
• Beneficiário: Aparecida Sadae Tanaka
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 11/09340-3 - Estudo funcional e estrutural de inibidores de proteases tipo cistatinas identificados em intestino do carrapato Rhipicephalus (Boophilus) microplus.
• Beneficiário: Thyago Hermylly Santana Cardoso
• Modalidade de apoio: Bolsas no Brasil - Doutorado