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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Characterization of a novel cystatin type 2 from Rhipicephalus microplus midgut

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Author(s):
Cardoso, Thyago H. S. ; Lu, Stephen ; Gonzalez, Boris R. G. ; Torquato, Ricardo J. S. ; Tanaka, Aparecida S.
Total Authors: 5
Document type: Journal article
Source: Biochimie; v. 140, p. 117-121, SEP 2017.
Web of Science Citations: 1
Abstract

The Rhipicephalus (Boophilus) microplus is an exclusive bovine ectoparasite responsible for the transmission of pathogens that decrease meat, leather and milk productions. Cattle vaccination is an alternative to control tick infestations, but the discovery of potential antigens is still a challenge for researchers. Recently, our group performed a midgut transcriptome of engorged R. microplus tick, and out of 800-ESTs sequences one cystatin-coding sequence was identified and named Rmcystatin-4. In order to understand the physiological role of Rmcystatin-4, the aim of this work was the expression, purification and functional characterization of a novel type 2 cystatin from the tick R. microplus. Rmcystatin-4 gene expression was identified mostly in tick midgut suggesting its possible role in blood digestion control. Our data showed that rRmcystatin-4 was successfully expressed in active form using Pichia pastoris system and the purified inhibitor presented high selectivity to BmCl-1 (Ki = 0.046 nM). Moreover, rRmcystatin-4 was able to impaired BmCl-1 activity towards bovine hemoglobin. (C) 2017 Elsevier B.V. and Societe Francaise de Biochimie et Biologie Moleculaire (SFBBM). All rights reserved. (AU)

FAPESP's process: 12/03657-8 - Inhibitor and proteases of ectoparasites: relationship of structure-function and identification of the role of these molecules in the interaction of diseases vector e their etiological agents
Grantee:Aparecida Sadae Tanaka
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 11/09340-3 - Functional and structural studies of protease inhibitor cistatin type identified in midgut of Rhipicephalus (Boophilus) microplus tick.
Grantee:Thyago Hermylly Santana Cardoso
Support Opportunities: Scholarships in Brazil - Doctorate