Abstract
The knowledge of the diversity of molecules produced by disease vectors in response to their etiologic agents is an important strategy to identify important targets in these vectors physiology, which could be used in rational design of drugs and pesticides. In attempt to contribute in this research field, this project has as general objective understand the role of protease inhibitors, proteolytic enzymes and hypothetical proteins identified in previous projects (using the following models of study: Triatoma infestans, one of the main vectors of Chagas Disease in Latin America, Aedes aegypti mosquito, vector of Dengue and the tick species Rhipicephalus (Boophilus) microplus, ectoparasite of cattle) in the interaction of these vectors with its etiological agents (Trypanosoma cruzi, dengue virus and Anaplasma marginale). The experience of the group in the research related to structure and function of protease inhibitors and anticoagulants (of the three vectors used in this study) will facilitate the implementation of the proposed project. To ensure the success of this project, the experiments of infection of the vector with their etiological agents will be carried out in collaboration with researchers who already have proven experience in the models of infection to be used. In order to achieve the desired results, the group are going to use the following tools: production of recombinant proteins and antibodies to these proteins, RNA interference, transcriptome, fluorescence microscopy, proteomic analysis, kinetic measurements, experiments in system of plasma resonance, immunization of cattle, experiments larvicides for Aedes and cell culture of tick and mosquito. The knowledge generated in this study will help in the understanding the role of protease inhibitors, proteases and other molecules in the vector-etiological agent interactions and also it will contribute to the formation of human resources and identification of molecules with biotechnological potential regarding problems related to livestock and public health. (AU)
Scientific publications
(16)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
OLIVEIRA, FELIPE A. A.;
BURI, MARCUS V.;
RODRIGUEZ, BORIS L.;
COSTA-DA-SILVA, ANDRE L.;
ARAUJO, HELENA R. C.;
CAPURRO, MARGARETH L.;
LU, STEPHEN;
TANAKA, APARECIDA S.
The first characterization of a cystatin and a cathepsin L-like peptidase from Aedes aegypti and their possible role in DENV infection by the modulation of apoptosis.
International Journal of Biological Macromolecules,
v. 146,
p. 141-149,
MAR 1 2020.
Web of Science Citations: 0.
SOARES, TATIANE SANCHES;
RODRIGUEZ GONZALEZ, BORIS LUIS;
SOARES TORQUATO, RICARDO JOSE;
ALVES LEMOS, FRANCISCO JOSE;
COSTA-DA-SILVA, ANDRE L.;
CAPURRO GUIMARAES, MARGARETH DE LARA;
TANAKA, APARECIDA SADAE.
Functional characterization of a serine protease inhibitor modulated in the infection of the Aedes aegypti with dengue virus.
Biochimie,
v. 144,
p. 160-168,
JAN 2018.
Web of Science Citations: 4.
GOMES, CICERA MARIA;
DE MORAIS-ZANI, KAREN;
LU, STEPHEN;
BUARQUE, DIEGO DE SOUZA;
CARDOSO BRAZ, GLORIA REGINA;
GREGO, KATHLEEN FERNANDES;
TANAKA, APARECIDA SADAE;
TANAKA-AZEVEDO, ANITA MITICO.
Differential transcript profile of inhibitors with potential anti-venom role in the liver of juvenile and adult Bothrops jararaca snake.
PeerJ,
v. 5,
APR 27 2017.
Web of Science Citations: 2.
BUARQUE, DIEGO S.;
GOMES, CICERA M.;
ARAUJO, RICARDO N.;
PEREIRA, MARCOS H.;
FERREIRA, ROBERTA C.;
GUARNERI, ALESSANDRA A.;
TANAKA, APARECIDA S.
A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota.
Biochimie,
v. 123,
p. 138-143,
APR 2016.
Web of Science Citations: 6.
SOARES, TATIANE S.;
OLIVEIRA, FELIPE;
TORQUATO, RICARDO J. S.;
SASAKI, SERGIO D.;
ARAUJO, MARIANA S.;
PASCHOALIN, THAYSA;
TANAKA, APARECIDA S.
BmTI-A, a Kunitz type inhibitor from Rhipicephalus microplus able to interfere in vessel formation.
Veterinary Parasitology,
v. 219,
p. 44-52,
MAR 30 2016.
Web of Science Citations: 7.
SOARES, TATIANE S.;
BUARQUE, DIEGO S.;
QUEIROZ, BRUNA R.;
GOMES, CICERA M.;
BRAZ, GLORIA R. C.;
ARAUJO, RICARDO N.;
PEREIRA, MARCOS H.;
GUARNERI, ALESSANDRA A.;
TANAKA, APARECIDA S.
A Kazal-type inhibitor is modulated by Trypanosoma cruzi to control microbiota inside the anterior midgut of Rhodnius prolixus.
Biochimie,
v. 112,
p. 41-48,
MAY 2015.
Web of Science Citations: 13.
ABREU, PATRICIA A.;
SOARES, TATIANE S.;
BUARQUE, DIEGO S.;
TORQUATO, RICARDO S.;
TANAKA, APARECIDA S.
RmKK, a tissue kallikrein inhibitor from Rhipicephalus microplus eggs.
Biochemical and Biophysical Research Communications,
v. 449,
n. 1,
p. 69-73,
JUN 20 2014.
Web of Science Citations: 3.
BUARQUE, DIEGO S.;
BRAZ, GLORIA R. C.;
MARTINS, RAFAEL M.;
TANAKA-AZEVEDO, ANITA M.;
GOMES, CICERA M.;
OLIVEIRA, FELIPE A. A.;
SCHENKMAN, SERGIO;
TANAKA, APARECIDA S.
Differential Expression Profiles in the Midgut of Triatoma infestans Infected with Trypanosoma cruzi.
PLoS One,
v. 8,
n. 5
MAY 2 2013.
Web of Science Citations: 14.