Advanced search
Start date
Betweenand
Related content
(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

The first characterization of a cystatin and a cathepsin L-like peptidase from Aedes aegypti and their possible role in DENV infection by the modulation of apoptosis

Full text
Author(s):
Oliveira, Felipe A. A. [1] ; Buri, Marcus V. [1] ; Rodriguez, Boris L. [1] ; Costa-da-Silva, Andre L. [2] ; Araujo, Helena R. C. [2] ; Capurro, Margareth L. [2] ; Lu, Stephen [1] ; Tanaka, Aparecida S. [1]
Total Authors: 8
Affiliation:
[1] Univ Fed Sao Paulo UNIFESP, Dept Biochem, Escola Paulista Med, Sao Paulo - Brazil
[2] Univ Sao Paulo, Inst Ciencias Biomed, Dept Parasitol, Sao Paulo - Brazil
Total Affiliations: 2
Document type: Journal article
Source: International Journal of Biological Macromolecules; v. 146, p. 141-149, MAR 1 2020.
Web of Science Citations: 0
Abstract

Recently, a salivary gland transcriptome study demonstrated that the transcripts of a putative cystatin gene (SeqID AAEL013287; Aacystatins) from Aedes aegypti were increased in DENV2-infected mosquitoes and that silencing of the Aacystatin gene resulted in an increase in DENV titres. In this work, Aacystatin was biochemically characterized; the purified recombinant inhibitor was able to inhibit typical cysteine proteases with a Ki in the nM range. Pulldown assays using Aag2 cell extracts identified a cathepsin L-like peptidase (AaCatL) as a possible target of Aacystatin. Purified recombinant AaCatL had an optimal pH of 5.0 and displayed a preference for Leu, Val and Phe residues at P2, which is common for other cathepsin L-like peptidases. Transcription analysis of Aacystatin and AaCatL in the salivary glands and midgut of DENV2-infected mosquitoes revealed a negative correlation between DENV2 titres and levels of the inhibitor and peptidase, suggesting their involvement in DENV2-mosquito interactions. Considering that apoptosis may play an important role during viral infections, the possible involvement of Aacystatin in staurosporine-induced apoptosis in Aag2 cells was investigated; the results showed higher expression of the inhibitor in treated cells; moreover, pre incubation with rAacystatin was able to increase Aag2 cell viability. (C) 2019 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 13/16609-4 - Aedes aegypti sex conversion by transgenesis
Grantee:Helena Rocha Corrêa de Araújo
Support type: Scholarships in Brazil - Post-Doctorate
FAPESP's process: 12/03657-8 - Inhibitor and proteases of ectoparasites: relationship of structure-function and identification of the role of these molecules in the interaction of diseases vector e their etiological agents
Grantee:Aparecida Sadae Tanaka
Support type: Research Projects - Thematic Grants