Short term changes in the proteome of human cerebral organoids induced by 5-MeO-DMT

Autor(es)

Dakic, Vanja ^{[1, 2]} ; Nascimento, Juliana Minardi ^{[2, 3]} ; Sartore, Rafaela Costa ^{[1, 2]} ; Maciel, Renata de Moraes ^[2] ; de Araujo, Draulio B. ^[4] ; Ribeiro, Sidarta ^[4] ; Martins-de-Souza, Daniel ^{[3, 5]} ; Rehen, Stevens K. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Rio de Janeiro, Inst Biomed Sci, Rio De Janeiro - Brazil

^[2] DOr Inst Res & Educ IDOR, Rio De Janeiro - Brazil

^[3] Univ Campinas UNICAMP, Dept Biochem & Tissue Biol, Inst Biol, Lab Neuroprote, Campinas, SP - Brazil

^[4] Univ Fed Rio Grande do Norte, Brain Inst, Natal, RN - Brazil

^[5] Conselho Nacl Desenvolvimento Cient & Tecnol, Inst Nacl Biomarcadores Neuropsiquiatria INBION, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 7, OCT 9 2017.
• Citações Web of Science: 12

Resumo

Dimethyltryptamines are entheogenic serotonin-like molecules present in traditional Amerindian medicine recently associated with cognitive gains, antidepressant effects, and changes in brain areas related to attention. Legal restrictions and the lack of adequate experimental models have limited the understanding of how such substances impact human brain metabolism. Here we used shotgun mass spectrometry to explore proteomic differences induced by 5-methoxy-N, N-dimethyltryptamine (5-MeO-DMT) on human cerebral organoids. Out of the 6,728 identified proteins, 934 were found differentially expressed in 5-MeO-DMT-treated cerebral organoids. In silico analysis reinforced previously reported anti-inflammatory actions of 5-MeO-DMT and revealed modulatory effects on proteins associated with long-term potentiation, the formation of dendritic spines, including those involved in cellular protrusion formation, microtubule dynamics, and cytoskeletal reorganization. Our data offer the first insight about molecular alterations caused by 5-MeO-DMT in human cerebral organoids. (AU)

• Processo FAPESP: 14/21035-0 - Proteômica quantitativa de linhagens neurais e organóides cerebrais derivados de células tronco de pluripotência induzida de pacientes com esquizofrenia
• Beneficiário: Juliana Minardi Nascimento
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 14/10068-4 - EMU concedido no processo 13/08711-3: espectrômetro de massas Waters SYNAPT G2-Si HDMs + nanoACQUITY UPLC
• Beneficiário: Daniel Martins-de-Souza
• Modalidade de apoio: Auxílio à Pesquisa - Programa Equipamentos Multiusuários
• Processo FAPESP: 13/08711-3 - Desenvolvimento de um teste preditivo para medicação bem sucedida e compreensão das bases moleculares da esquizofrenia através da proteômica
• Beneficiário: Daniel Martins-de-Souza
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores