Bioremediation of cooking oil waste using lipases from wastes

Autor(es)

Okino-Delgado, Clarissa Hamaio ^[1] ; do Prado, Debora Zanoni ^[1] ; Facanali, Roselaine ^[2] ; Ortiz Marques, Marcia Mayo ^[2] ; Nascimento, Augusto Santana ^[1] ; da Costa Fernandes, Celio Junior ^[1] ; Zambuzzi, William Fernando ^[1] ; Fleuri, Luciana Francisco ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Chem & Biochem Dept, Botucatu, SP - Brazil

^[2] CEP, Agron Inst IAC, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: PLoS One; v. 12, n. 10 OCT 26 2017.
• Citações Web of Science: 10

Resumo

Cooking oil waste leads to well-known environmental impacts and its bioremediation by lipase-based enzymatic activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, although lipases are able to process cooking oil wastes, the products generated from this process do not necessarily become less toxic. Thus, the aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. Thus, assessed the post-frying modification in soybean oil and in its waste, which was caused by hydrolysis reaction catalyzed by commercial and home-made lipases. The presence of lipases in the extracts obtained from orange wastes was identified by zymography. The profile of the fatty acid esters formed after these reactions was detected and quantified through gas chromatography and fatty acids profile compared through multivariate statistical analyses. Finally, the soybean oil and its waste, with and without enzymatic treatment, were assessed for toxicity in cytotoxicity assays conducted in vitro using fibroblast cell culture. The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases. (AU)

• Processo FAPESP: 15/01753-8 - Resíduos agroindustriais como fontes indiretas e diretas de lipases: obtenção a baixo custo, aplicações em reações de hidrólise e síntese e correlações estruturais com atividades biológicas
• Beneficiário: Luciana Francisco Fleuri
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/22689-3 - Sinalização parácrina mediada por microvesículas e proteínas entre células ósseas e endoteliais durante o desenvolvimento e regeneração do tecido ósseo
• Beneficiário: Willian Fernando Zambuzzi
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores
• Processo FAPESP: 14/10962-7 - Desenvolvimento de biocatalisadores a partir de resíduos vegetais para aplicação em biorremediação e produção de biodiesel
• Beneficiário: Clarissa Hamaio Okino Delgado
• Modalidade de apoio: Bolsas no Brasil - Doutorado