Bioremediation of cooking oil waste using lipases from wastes

Author(s)

Okino-Delgado, Clarissa Hamaio ^[1] ; do Prado, Debora Zanoni ^[1] ; Facanali, Roselaine ^[2] ; Ortiz Marques, Marcia Mayo ^[2] ; Nascimento, Augusto Santana ^[1] ; da Costa Fernandes, Celio Junior ^[1] ; Zambuzzi, William Fernando ^[1] ; Fleuri, Luciana Francisco ^[1]

Affiliation

^[1] Sao Paulo State Univ UNESP, Inst Biosci, Chem & Biochem Dept, Botucatu, SP - Brazil

^[2] CEP, Agron Inst IAC, Campinas, SP - Brazil

• Document type: Journal article
• Source: PLoS One; v. 12, n. 10 OCT 26 2017.
• Web of Science Citations: 10

Abstract

Cooking oil waste leads to well-known environmental impacts and its bioremediation by lipase-based enzymatic activity can minimize the high cytotoxic potential. In addition, they are among the biocatalysts most commercialized worldwide due to the versatility of reactions and substrates. However, although lipases are able to process cooking oil wastes, the products generated from this process do not necessarily become less toxic. Thus, the aim of the current study is to analyze the bioremediation of lipase-catalyzed cooking oil wastes, as well as their effect on the cytotoxicity of both the oil and its waste before and after enzymatic treatment. Thus, assessed the post-frying modification in soybean oil and in its waste, which was caused by hydrolysis reaction catalyzed by commercial and home-made lipases. The presence of lipases in the extracts obtained from orange wastes was identified by zymography. The profile of the fatty acid esters formed after these reactions was detected and quantified through gas chromatography and fatty acids profile compared through multivariate statistical analyses. Finally, the soybean oil and its waste, with and without enzymatic treatment, were assessed for toxicity in cytotoxicity assays conducted in vitro using fibroblast cell culture. The soybean oil wastes treated with core and frit lipases through transesterification reaction were less toxic than the untreated oils, thus confirming that cooking oil wastes can be bioremediated using orange lipases. (AU)

• FAPESP's process: 15/01753-8 - Agroindustrial residues as indirect and direct sources of lipases: obtaining at low cost, applications in hydrolysis and synthesis reactions and structural correlations with biological activities
• Grantee: Luciana Francisco Fleuri
• Support Opportunities: Regular Research Grants
• FAPESP's process: 14/22689-3 - Microvesicle/proteins-mediated paracrine signaling among bone and endothelial cells during bone development and regeneration
• Grantee: Willian Fernando Zambuzzi
• Support Opportunities: Research Grants - Young Investigators Grants
• FAPESP's process: 14/10962-7 - Development of biocatalysts from plant wastes for application in bioremediation and biodiesel production
• Grantee: Clarissa Hamaio Okino Delgado
• Support Opportunities: Scholarships in Brazil - Doctorate