Environmental DNA characterization of amphibian communities in the Brazilian Atlantic forest: Potential application for conservation of a rich and threatened fauna

Autor(es)

Sasso, Thais ^[1] ; Lopes, Carla M. ^{[2, 3]} ; Valentini, Alice ^[4] ; Dejean, Tony ^[4] ; Zarnudio, Kelly R. ^[5] ; Haddad, Celio F. B. ^{[2, 3]} ; Martins, Marcio ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biociencias, Dept Ecol, Rua Matao, Trav 321, BR-05508090 Sao Paulo, SP - Brazil

^[2] Univ Estadual Paulista UNESP, Dept Zool, Inst Biociencias, Campus Rio Claro, BR-13506900 Rio Claro, SP - Brazil

^[3] Ctr Aquicultura CAUNESP, Campus Rio Claro, BR-13506900 Rio Claro, SP - Brazil

^[4] SPYGEN, Savoie Technolac, BP 274, F-73375 Le Bourget Du Lac - France

^[5] Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 - USA

• Tipo de documento: Artigo Científico
• Fonte: Biological Conservation; v. 215, p. 225-232, NOV 2017.
• Citações Web of Science: 2

Resumo

The Brazilian Atlantic forest is a highly threatened biodiversity hotspot, harboring one of the highest levels of amphibian species richness in the world. Amphibian conservation in Neotropical biomes is critical because freshwater ecosystems typically experience sharp declines in biodiversity before much is known about species that depend on those environments. Environmental DNA (eDNA) analysis is a new approach for monitoring aquatic organisms and provides valuable information on species occurrence in freshwater ecosystems. Here, we assess community diversity in Neotropical streams using eDNA metabarcoding. We compare data from a five-year traditional field survey with results from a short-term eDNA analysis in four streams of the Atlantic forest of southeastern Brazil. We recorded 19 species over 5 years using visual-acoustic methods, of which 10 species are associated with the streams during at least one life stage (egg, tadpole or post-metamorphic). We were able to detect nine of the latter species using eDNA metabarcoding from water samples collected over 4 days. Amphibian community composition as measured by both methods showed high similarity in three streams, but was not perfect, as eDNA failed to detect known species in a fourth stream. Furthermore, in one stream we detected through eDNA metabarcoding a species (Aplastodiscus eugenioi) found only once during the 5-year traditional survey in that stream. Also, three species (Cycloramphus boraceiensis, Hylodes asper, and Hylodes phyllodes) with the highest dependence on aquatic habitat showed the highest number of positive PCR detections on eDNA samples. Our results showed that eDNA metabarcoding can be a useful tool to assess community diversity in tropical streams, and although not perfect in detection, this method can potentially improve our ability to conserve Neotropical amphibians. (AU)

• Processo FAPESP: 06/58011-4 - Conservacao de anfibios e repteis neotropicais: aspectos ecologicos e evolutivos.
• Beneficiário: Marcio Roberto Costa Martins
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 10/50146-3 - Padrões de história natural e distribuição de anfíbios e répteis como base para estudos de ecologia, evolução e conservação
• Beneficiário: Marcio Roberto Costa Martins
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/14054-3 - DNA ambiental aplicado a estudos de monitoramento e conservação de anfíbios anuros na Mata Atlântica
• Beneficiário: Carla Martins Lopes
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 13/50741-7 - Diversidade e conservação dos anfíbios brasileiros
• Beneficiário: Célio Fernando Baptista Haddad
• Modalidade de apoio: Auxílio à Pesquisa - Programa BIOTA - Temático
• Processo FAPESP: 14/06795-8 - Detecção de espécies de anuros em riachos de Mata Atlântica por meio de DNA ambiental
• Beneficiário: Thais Sasso Lopes
• Modalidade de apoio: Bolsas no Brasil - Mestrado