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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Environmental DNA characterization of amphibian communities in the Brazilian Atlantic forest: Potential application for conservation of a rich and threatened fauna

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Autor(es):
Sasso, Thais [1] ; Lopes, Carla M. [2, 3] ; Valentini, Alice [4] ; Dejean, Tony [4] ; Zarnudio, Kelly R. [5] ; Haddad, Celio F. B. [2, 3] ; Martins, Marcio [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Sao Paulo, Inst Biociencias, Dept Ecol, Rua Matao, Trav 321, BR-05508090 Sao Paulo, SP - Brazil
[2] Univ Estadual Paulista UNESP, Dept Zool, Inst Biociencias, Campus Rio Claro, BR-13506900 Rio Claro, SP - Brazil
[3] Ctr Aquicultura CAUNESP, Campus Rio Claro, BR-13506900 Rio Claro, SP - Brazil
[4] SPYGEN, Savoie Technolac, BP 274, F-73375 Le Bourget Du Lac - France
[5] Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 - USA
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Biological Conservation; v. 215, p. 225-232, NOV 2017.
Citações Web of Science: 2
Resumo

The Brazilian Atlantic forest is a highly threatened biodiversity hotspot, harboring one of the highest levels of amphibian species richness in the world. Amphibian conservation in Neotropical biomes is critical because freshwater ecosystems typically experience sharp declines in biodiversity before much is known about species that depend on those environments. Environmental DNA (eDNA) analysis is a new approach for monitoring aquatic organisms and provides valuable information on species occurrence in freshwater ecosystems. Here, we assess community diversity in Neotropical streams using eDNA metabarcoding. We compare data from a five-year traditional field survey with results from a short-term eDNA analysis in four streams of the Atlantic forest of southeastern Brazil. We recorded 19 species over 5 years using visual-acoustic methods, of which 10 species are associated with the streams during at least one life stage (egg, tadpole or post-metamorphic). We were able to detect nine of the latter species using eDNA metabarcoding from water samples collected over 4 days. Amphibian community composition as measured by both methods showed high similarity in three streams, but was not perfect, as eDNA failed to detect known species in a fourth stream. Furthermore, in one stream we detected through eDNA metabarcoding a species (Aplastodiscus eugenioi) found only once during the 5-year traditional survey in that stream. Also, three species (Cycloramphus boraceiensis, Hylodes asper, and Hylodes phyllodes) with the highest dependence on aquatic habitat showed the highest number of positive PCR detections on eDNA samples. Our results showed that eDNA metabarcoding can be a useful tool to assess community diversity in tropical streams, and although not perfect in detection, this method can potentially improve our ability to conserve Neotropical amphibians. (AU)

Processo FAPESP: 10/50146-3 - Padrões de história natural e distribuição de anfíbios e répteis como base para estudos de ecologia, evolução e conservação
Beneficiário:Marcio Roberto Costa Martins
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 06/58011-4 - Conservacao de anfibios e repteis neotropicais: aspectos ecologicos e evolutivos.
Beneficiário:Marcio Roberto Costa Martins
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/50741-7 - Diversidade e conservação dos anfíbios brasileiros
Beneficiário:Célio Fernando Baptista Haddad
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático
Processo FAPESP: 14/06795-8 - Detecção de espécies de anuros em riachos de Mata Atlântica por meio de DNA ambiental
Beneficiário:Thais Sasso Lopes
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 16/14054-3 - DNA ambiental aplicado a estudos de monitoramento e conservação de anfíbios anuros na Mata Atlântica
Beneficiário:Carla Martins Lopes
Linha de fomento: Bolsas no Brasil - Pós-Doutorado