Proteomic study revealed cellular assembly and lipid metabolism dysregulation in sepsis secondary to community-acquired pneumonia

Autor(es)

Sharma, Narendra Kumar ^[1] ; Tashima, Alexandre Keiji ^[2] ; Colo Brunialti, Milena Karina ^[1] ; Ferreira, Eden Ramalho ^[3] ; Soares Torquato, Ricardo Jose ^[2] ; Mortara, Renato Arruda ^[3] ; Machado, Flavia Ribeiro ^[4] ; Assuncao, Murillo ^[5] ; Rigato, Otelo ^{[1, 6]} ; Salomao, Reinaldo ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Hosp Sao Paulo, Div Infect Dis, Escola Paulista Med, BR-04039032 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Dept Biochem, Escola Paulista Med, BR-04023900 Sao Paulo, SP - Brazil

^[3] Univ Fed Sao Paulo, Dept Microbiol Immunol & Parasitol, Escola Paulista Med, BR-04023062 Sao Paulo - Brazil

^[4] Univ Fed Sao Paulo, Intens Care Unit, Hosp Sao Paulo, Escola Paulista Med, BR-04024002 Sao Paulo - Brazil

^[5] Hosp Israelita Albert Einstein, Intens Care Unit, BR-05652900 Sao Paulo - Brazil

^[6] Hosp Sirio Libanes, Intens Care Unit, BR-01409001 Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: SCIENTIFIC REPORTS; v. 7, NOV 15 2017.
• Citações Web of Science: 4

Resumo

Sepsis is a life-threatening disorder characterized by organ dysfunction and a major cause of mortality worldwide. The major challenge in studying sepsis is its diversity in such factors as age, source of infection and etiology. Recently, genomic and proteomic approaches have improved our understanding of its complex pathogenesis. In the present study, we use quantitative proteomics to evaluate the host proteome response in septic patients secondary to community-acquired pneumonia (CAP). Samples obtained at admission and after 7 days of follow-up were analyzed according to the outcomes of septic patients. The patients' proteome profiles were compared with age-and gender-matched healthy volunteers. Bioinformatic analyses of differentially expressed proteins showed alteration in the cytoskeleton, cellular assembly, movement, lipid metabolism and immune responses in septic patients. Actin and gelsolin changes were assessed in mononuclear cells using immunofluorescence, and a higher expression of gelsolin and depletion of actin were observed in survivor patients. Regarding lipid metabolism, changes in cholesterol, HDL and apolipoproteins were confirmed using enzymatic colorimetric methods in plasma. Transcriptomic studies revealed a massive change in gene expression in sepsis. Our proteomic results stressed important changes in cellular structure and metabolism, which are possible targets for future interventions of sepsis. (AU)

• Processo FAPESP: 11/20401-4 - Sepse: integrando a pesquisa básica e a investigação clínica II
• Beneficiário: Reinaldo Salomão
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 13/15636-8 - Estudo de proteômica em pacientes com Sepse
• Beneficiário: Narendra Kumar Sharma
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado