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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Histologic chorioamnionitis does not modulate the oxidative stress and antioxidant status in pregnancies complicated by spontaneous preterm delivery

Texto completo
Autor(es):
Martin, Laura Fernandes [1] ; Moco, Natalia Prearo [1] ; de Lima, Moises Diogo [2] ; Polettini, Jossimara [3] ; Miot, Helio Amante [1] ; Correa, Camila Renata [1] ; Menon, Ramkumar [4] ; da Silva, Marcia Guimaraes [1]
Número total de Autores: 8
Afiliação do(s) autor(es):
[1] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Pathol, BR-18618686 Botucatu, SP - Brazil
[2] Univ Fed Paraiba, UFPB, Dept Gynecol & Obstet, Joao Pessoa, Paraiba - Brazil
[3] Univ Western Sao Paulo, UNOESTE, Presidente Prudente - Brazil
[4] Univ Texas Med Branch, Dept Obstet & Gynecol, Galveston, TX 77555 - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: BMC PREGNANCY AND CHILDBIRTH; v. 17, NOV 13 2017.
Citações Web of Science: 2
Resumo

Background: Infection induced-inflammation and other risk factors for spontaneous preterm birth (PTB) and preterm premature rupture of membranes (pPROM) may cause a redox imbalance, increasing the release of free radicals and consuming antioxidant defenses. Oxidative stress, in turn, can initiate intracellular signaling cascades that increase the production of pro-inflammatory mediators. The objective of this study was to evaluate the oxidative damage to proteins and antioxidant capacity profiles in amniochorion membranes from preterm birth (PTB) and preterm premature rupture of membranes (pPROM) and to determine the role of histologic chorioamnionitis in this scenario. Methods: We included 27 pregnant women with PTB, 27 pPROM and 30 at term. Protein oxidative damage was assayed by 3-nitrotyrosine (3-NT) and carbonyl levels, using enzyme-linked immunosorbent assay (ELISA) and modified dinitrophenylhydrazine assay (DNPH), respectively. Total antioxidant capacity (TAC) was measured by ELISA. Results: Protein oxidative damage determined by carbonyl levels was lower in PTB group than pPROM and term groups (p < 0.001). PTB group presented higher TAC compared with pPROM and term groups (p = 0.002). Histologic chorioamnionitis did not change either protein oxidative damage or TAC regardless of gestational outcome. Conclusion: These results corroborates previous reports that pPROM and term birth exhibit similarities in oxidative stress-induced senescence and histologic chorioamnionitis does not modulate oxidative stress or antioxidant status. (AU)

Processo FAPESP: 12/17234-1 - Estudo in vitro de infecção das membranas corioamnióticas com espécies bacterianas isoladas do trato genital inferior e modulação da resposta imune inata na interface materno-fetal
Beneficiário:Márcia Guimarães da Silva
Modalidade de apoio: Auxílio à Pesquisa - Regular