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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

eDNA metabarcoding: a promising method for anuran surveys in highly diverse tropical forests

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Autor(es):
Lopes, Carla M. [1] ; Sasso, Thais [2] ; Valentini, Alice [3] ; Dejean, Tony [3] ; Martins, Marcio [2] ; Zamudio, Kelly R. [4] ; Haddad, Celio F. B. [1]
Número total de Autores: 7
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista, Inst Biociencias, Dept Zool, Ave 24A, 1515, BR-13506900 Rio Claro, SP - Brazil
[2] Univ Sao Paulo, Inst Biociencias, Dept Ecol, BR-05508090 Sao Paulo, SP - Brazil
[3] Savoie Technolac, SPYGEN, BP 274, F-73375 Le Bourget Du Lac - France
[4] Cornell Univ, Dept Ecol & Evolutionary Biol, Ithaca, NY 14853 - USA
Número total de Afiliações: 4
Tipo de documento: Artigo Científico
Fonte: MOLECULAR ECOLOGY RESOURCES; v. 17, n. 5, p. 904-914, SEP 2017.
Citações Web of Science: 15
Resumo

Understanding the geographical distribution and community composition of species is crucial to monitor species persistence and define effective conservation strategies. Environmental DNA (eDNA) has emerged as a powerful noninvasive tool for species detection. However, most eDNA survey methods have been developed and applied in temperate zones. We tested the feasibility of using eDNA to survey anurans in tropical streams in the Brazilian Atlantic forest and compared the results with short-term visual and audio surveys. We detected all nine species known to inhabit our focal streams with one single visit for eDNA sampling. We found a higher proportion of sequence reads and larger number of positive PCR replicates for more common species and for those with life cycles closely associated with the streams, factors that may contribute to increased release of DNA in the water. However, less common species were also detected in eDNA samples, demonstrating the detection power of this method. Filtering larger volumes of water resulted in a higher probability of detection. Our data also show it is important to sample multiple sites along streams, particularly for detection of target species with lower population densities. For the three focal species in our study, the eDNA metabarcoding method had a greater capacity of detection per sampling event than our rapid field surveys, and thus, has the potential to circumvent some of the challenges associated with traditional approaches. Our results underscore the utility of eDNA metabarcoding as an efficient method to survey anuran species in tropical streams of the highly biodiverse Brazilian Atlantic forest. (AU)

Processo FAPESP: 16/14054-3 - DNA ambiental aplicado a estudos de monitoramento e conservação de anfíbios anuros na Mata Atlântica
Beneficiário:Carla Martins Lopes
Linha de fomento: Bolsas no Brasil - Pós-Doutorado
Processo FAPESP: 13/50741-7 - Diversidade e conservação dos anfíbios brasileiros
Beneficiário:Célio Fernando Baptista Haddad
Linha de fomento: Auxílio à Pesquisa - Programa BIOTA - Temático