Proteome changes of beef in Nellore cattle with different genotypes for tenderness

Autor(es)

Rosa, Alessandra F. ^[1] ; Moncau, Cristina T. ^[1] ; Poleti, Mirele D. ^{[1, 2]} ; Fonseca, Leydiana D. ^[1] ; Balieiro, Julio C. C. ^{[1, 3]} ; Silva, Saulo L. E. ^[4] ; Eler, Joanir P. ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Dept Vet Med, Coll Anim Sci & Food Engn, 225 Duque de Caxias, BR-13635900 Pirasssurumga, SP - Brazil

^[2] Univ Sao Paulo, Luiz de Queiroz Coll Agr, Dept Anim Sci, Padua Dias 11, BR-13418900 Piracicaba, SP - Brazil

^[3] Univ Sao Paulo, Sch Vet Med & Anim Sci, Dept Anim Nutr & Prod, 225 Duque de Caxias, BR-13635900 Pirassununga, SP - Brazil

^[4] Univ Sao Paulo, Coll Anim Sci & Food Engn, Dept Anim Sci, 225 Duque de Caxias, BR-13635900 Pirasssununga, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: MEAT SCIENCE; v. 138, p. 1-9, APR 2018.
• Citações Web of Science: 9

Resumo

In the present study, 155 Nellore cattle were genotyped for the CAPN4751 and UOGCAST polymorphisms and phenotyped for shear force (SF) at 1, 7 and 14 days aging. The effects of different genotypic combinations were evaluated on the Longissimus muscle proteomic profile using 2DE and mass spectrometry. A significant association was found between genotypes for UOGCAST and CAPN4751 and meat tenderness. The CC genotype for both markers was favorable for lesser shear force than TT. A total of 40 spots showed significant differential expression profiles (P < 0.05), of which eight had a main effect for the CAPN4751 marker, 11 for UOGCAST, two for both markers, and 19 had interactions between markers, including myosin (MYL1, MYL2, MYLPF and MYL6B), actin (ACTA1 and CAPZ beta), troponin (TNNT1 and TNNT3) and heat shock proteins (HSPB6, HSPB1 and HSP70-2). The results demonstrated that UOGCAST and CAPN4751 genotypes led to variability on the expression of proteins that are involved in muscle metabolism, and consequently affect meat tenderness. (AU)

• Processo FAPESP: 08/09938-3 - Análise proteômica do efeito do complexo calpaína/calpastatina na carne de bovinos nelore com diferentes combinações genotípicas para maciez
• Beneficiário: Alessandra Fernandes Rosa
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado