Design and characterization of crotamine-functionalized gold nanoparticles

Autor(es):Mostrar menos -

Karpel, Richard L. ^[1] ; Liberato, Michelle da Silva ^[2] ; Campeiro, Joana D. ^[3] ; Bergeon, Lorna ^[1] ; Szychowski, Brian ^[1] ; Butler, Andrew ^[1] ; Marino, Giovanni ^[1] ; Cusic, Joelle F. ^[1] ; Goncalves de Oliveira, Lilian Caroline ^[4] ; Oliveira, Eduardo B. ^[5] ; de Farias, Marcelo Alexandre ^[6] ; Portugal, Rodrigo Villares ^[6] ; Alves, Wendel Andrade ^[2] ; Daniel, Marie-Christine ^[1] ; Hayashi, Mirian A. F. ^[3]

Afiliação do(s) autor(es)

^[1] UMBC, Dept Chem & Biochem, 1000 Hilltop Circle, Baltimore, MD 21250 - USA

^[2] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil

^[3] Univ Fed Sao Paulo UNIFESP, EPM, Dept Farmacol, Sao Paulo, SP - Brazil

^[4] Univ Fed Sao Paulo UNIFESP, EPM, Dept Biofis, Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo USP RP, Dept Bioquim & Imunol, Ribeirao Preto, SP - Brazil

^[6] CNPEM, Brazilian Nanotechnol Natl Lab LNNano, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 163, p. 1-8, MAR 1 2018.
• Citações Web of Science: 3

Resumo

This paper describes the development of a facile and environmentally friendly strategy for supporting crotamine on gold nanoparticles (GNPs). Our approach was based on the covalent binding interaction between the cell penetrating peptide crotamine, which is a snake venom polypeptide with preference to penetrate dividing cells, and a polyethylene glycol (PEG) ligand, which is a nontoxic, water-soluble and easily obtainable commercial polymer. Crotamine was derivatized with ortho-pyridyldisulfide-polyethyleneglycol-N-hydroxysuccinimide (OPSS-PEG-SVA) cross-linker to produce OPSS-PEG-crotamine as the surface modifier of GNP. OPSS-PEG-SVA can serve not only as a surface modifier, but also as a stabilizing agent for GNPs. The successful PEGylation of the nanoparticles was demonstrated using different physicochemical techniques, while the grafting densities of the PEG ligands and crotamine on the surface of the nanoparticles were estimated using a combination of electron microscopy and mass spectrometry analysis. In vitro assays confirmed the internalization of these GNPs, into living HeLa cells. The results described herein suggest that our approach may serve as a simple platform for the synthesis Of GNPs decorated with crotamine with well-defined morphologies and uniform dispersion, opening new roads for crotamine biomedical applications. (C) 2017 Elsevier B.V. All rights reserved. (AU)

• Processo FAPESP: 15/24018-1 - Conjugados polímero-peptídeo para formulação de hidrogéis e seu uso em detecção
• Beneficiário: Wendel Andrade Alves
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 13/13392-4 - Avaliação do uso dos análogos da crotamina em diagnóstico ou terapia
• Beneficiário: Mirian Akemi Furuie Hayashi
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/02413-1 - Validação da crotamina como biomarcador e avaliação do seu potencial uso na terapia de doenças humanas
• Beneficiário: Mirian Akemi Furuie Hayashi
• Modalidade de apoio: Auxílio à Pesquisa - Regular