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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Design and characterization of crotamine-functionalized gold nanoparticles

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Author(s):
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Karpel, Richard L. [1] ; Liberato, Michelle da Silva [2] ; Campeiro, Joana D. [3] ; Bergeon, Lorna [1] ; Szychowski, Brian [1] ; Butler, Andrew [1] ; Marino, Giovanni [1] ; Cusic, Joelle F. [1] ; Goncalves de Oliveira, Lilian Caroline [4] ; Oliveira, Eduardo B. [5] ; de Farias, Marcelo Alexandre [6] ; Portugal, Rodrigo Villares [6] ; Alves, Wendel Andrade [2] ; Daniel, Marie-Christine [1] ; Hayashi, Mirian A. F. [3]
Total Authors: 15
Affiliation:
[1] UMBC, Dept Chem & Biochem, 1000 Hilltop Circle, Baltimore, MD 21250 - USA
[2] Univ Fed ABC, Ctr Ciencias Nat & Humanas, Santo Andre, SP - Brazil
[3] Univ Fed Sao Paulo UNIFESP, EPM, Dept Farmacol, Sao Paulo, SP - Brazil
[4] Univ Fed Sao Paulo UNIFESP, EPM, Dept Biofis, Sao Paulo, SP - Brazil
[5] Univ Sao Paulo USP RP, Dept Bioquim & Imunol, Ribeirao Preto, SP - Brazil
[6] CNPEM, Brazilian Nanotechnol Natl Lab LNNano, Campinas, SP - Brazil
Total Affiliations: 6
Document type: Journal article
Source: COLLOIDS AND SURFACES B-BIOINTERFACES; v. 163, p. 1-8, MAR 1 2018.
Web of Science Citations: 3
Abstract

This paper describes the development of a facile and environmentally friendly strategy for supporting crotamine on gold nanoparticles (GNPs). Our approach was based on the covalent binding interaction between the cell penetrating peptide crotamine, which is a snake venom polypeptide with preference to penetrate dividing cells, and a polyethylene glycol (PEG) ligand, which is a nontoxic, water-soluble and easily obtainable commercial polymer. Crotamine was derivatized with ortho-pyridyldisulfide-polyethyleneglycol-N-hydroxysuccinimide (OPSS-PEG-SVA) cross-linker to produce OPSS-PEG-crotamine as the surface modifier of GNP. OPSS-PEG-SVA can serve not only as a surface modifier, but also as a stabilizing agent for GNPs. The successful PEGylation of the nanoparticles was demonstrated using different physicochemical techniques, while the grafting densities of the PEG ligands and crotamine on the surface of the nanoparticles were estimated using a combination of electron microscopy and mass spectrometry analysis. In vitro assays confirmed the internalization of these GNPs, into living HeLa cells. The results described herein suggest that our approach may serve as a simple platform for the synthesis Of GNPs decorated with crotamine with well-defined morphologies and uniform dispersion, opening new roads for crotamine biomedical applications. (C) 2017 Elsevier B.V. All rights reserved. (AU)

FAPESP's process: 15/24018-1 - Polymer-Peptide Conjugates for hydrogel formulation and its usage in detection
Grantee:Wendel Andrade Alves
Support Opportunities: Regular Research Grants
FAPESP's process: 13/13392-4 - Evaluation of the use of analogs of crotamine for diagnosis or therapy
Grantee:Mirian Akemi Furuie Hayashi
Support Opportunities: Regular Research Grants
FAPESP's process: 17/02413-1 - Validation of crotamine as a biomarker and evaluation of its potential use in the therapy of human diseases
Grantee:Mirian Akemi Furuie Hayashi
Support Opportunities: Regular Research Grants