Proanthocyanidin polymeric tannins from Stryphnodendron adstringens are effective against Candida spp. isolates and for vaginal candidiasis treatment

Autor(es)

Duarte de Freitas, Aline Luiza ^[1] ; Kaplum, Vanessa ^[2] ; Pereira Rossi, Diego Conrado ^[1] ; Roque da Silva, Leandro Buffoni ^[3] ; Carvalho Melhem, Marcia de Souza ^[4] ; Taborda, Carlos Pelleschi ^{[1, 3]} ; Palazzo de Mello, Joao Carlos ^[5] ; Nakamura, Celso Vataru ; Ishida, Kelly ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Ciencias Biomed, Dept Microbiol, Sao Paulo, SP - Brazil

^[2] Univ Estadual Maringa, Dept Ciencias Basicas Saude, Maringa, Parana - Brazil

^[3] Univ Sao Paulo, Trop Med Inst USP LIM53, Lab Med Mycol, Sao Paulo - Brazil

^[4] Adolfo Lutz Inst, Sao Paulo, SP - Brazil

^[5] Univ Estadual Maringa, Dept Farm, Maringa, Parana - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Journal of Ethnopharmacology; v. 216, p. 184-190, APR 24 2018.
• Citações Web of Science: 5

Resumo

Ethnopharmacological relevance: The stem bark of Stryphnodendron adstringens (Mart.) Coville is popularly used as anti-inflammatory, astringent and in the treatment of wounds and vaginal infections. Several pharmacological activities have been scientifically proven by in vitro and in vivo experimental assays for antibacterial, antiviral, antiprotozoan, anti-inflammatory and antioxidant. Aim of the study: We investigated whether proanthocyanidin polymeric tannins from the Stryphnodendron adstringens stem bark with antifungal activity against Candida albicans in vitro are also active against planktonic and biofilm cells of Candida non-albicans (CNA, including fluconazole-resistant isolates) and are capable of controlling Candida vaginitis in vivo. Materials and methods: A total of 46 clinical isolates and 5 reference Candida spp. strains were used in this study. The antifungal effects in vitro of tannins (F2 and sub-fraction F2.4) from S. adstringens stem bark were evaluated using a broth microdilution assay (for planktonic yeasts and biofilm dispersion cells) or by XTT assay (for biofilm sessile cells). For in vivo antifungal activity analysis, mice with vaginal infection by C. albicans or C. glabrata were treated with a topical gel containing F2 (alone or combined with oral fluconazole), and the vaginal histopathology and fungal burden (by CFU counts from vaginal homogenates) were analyzed. Results: F2 and F2.4 inhibited the proliferation of planktonic cells of Candida spp., especially that of fluconazoleand/or amphotericin B-resistant isolates. F2 and F2.4 also inhibited the proliferation of Candida biofilm dispersion cells. Moreover, a gel containing F2 efficiently controlled vaginal infection by C. albicans and C. glabrata in mice, with no noticeable toxicity to vaginal tissue. Conclusions: Our data show that proanthocyanidin polymeric tannins obtained from S. adstringens have anti fungal activity in vitro against C. albicans and CNA (including fluconazole-resistant isolates) and presented efficacy in the control of candidiasis in murine model. Therefore, these tannins have potential use in the treatment of vaginal candidiasis, representing interesting alternatives to current antifungals. (AU)

• Processo FAPESP: 16/04816-3 - Efeito inibitório de taninos hexaméricos de proantocianidinas obtidos das cascas de Stryphnodendron adstringens sobre biofilmes de espécies de Candida não-albicans
• Beneficiário: Aline Luiza Duarte de Freitas
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/11232-0 - Efeito farmacológico dos taninos condensados extraídos de Stryphnodendron adstringens: ação antibiofilme de Candida albicans, imunomodulatória e antifúngica em modelo murino de candidíase vaginal
• Beneficiário: Kelly Ishida
• Modalidade de apoio: Auxílio à Pesquisa - Regular