Induction of systemic inflammation by hyaluronan and hsp70 in women with pre-eclampsia

Autor(es)

Romao-Veiga, Mariana ^[1] ; Matias, Mariana Leticia ^[2] ; Ribeiro, Vanessa Rocha ^[2] ; Nunes, Priscila Rezeck ^[2] ; Borges, Vera Therezinha M. ^[2] ; Peracoli, Jose Carlos ^[2] ; Peracoli, Maria Terezinha S. ^[1]

Afiliação do(s) autor(es)

^[1] Sao Paulo State Univ, UNESP, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP - Brazil

^[2] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Gynaecol & Obstet, Botucatu, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CYTOKINE; v. 105, p. 23-31, MAY 2018.
• Citações Web of Science: 6

Resumo

Pre-eclampsia (PE) is a human pregnancy syndrome with abnormal activation of the innate immune response. The study evaluated the involvement of molecular structures called damage-associated molecular patterns (DAMPs), such as hyaluronan (HA) and heat shock proteins (Hsp) on NLRP1 and NLRP3 inflammasomes activation in peripheral blood monocytes. Twenty pre-eclamptic women, 20 normotensive pregnant women (NT) and 20 non-pregnant women (NP) were studied. Enzyme immunoassay was employed for the determination of HA, Hsp70 and High mobility group Box 1 (HMGB1) in plasma, as well as for the detection of Interleukin-1 beta (IL-1 beta), IL-18 and tumor necrosis factor alpha (TNF-alpha) in the supernatant of monocytes cultured with or without HA and Hsp70. The inflammasomes induction was evaluated by the quantification of mRNA for NLRP1, NLRP3, caspase-1, IL-1 beta, IL-18, HMGB1 and TNF-alpha by qPCR in monocyte culture. The results showed significantly higher plasma levels of HA, Hsp70 and HMGB1 in pre-eclamptic women than in NT and NP women. Monocytes from women with PE showed endogenous activation of NLRP1 and NLRP3 inflammasomes, and expressed high amounts of IL-1 beta, IL-18, HMGB1 and TNF-alpha. The stimulation of monocytes with HA increased the gene expression of NLRP1, NLRP3, caspase-1, TNF-alpha, HMGB1 and IL-18 and the production of IL-1 beta in preeclamptic women. Monocytes cultured with Hsp70 produced elevated levels of IL-1 beta and TNF-alpha through a mechanism independent of inflammasomes activation. These results suggest the participation of these DAMPs in the systemic inflammatory response that is characteristic of PE. (AU)

• Processo FAPESP: 13/00534-5 - Envolvimento do inflamassomo na imunidade inata e adaptativa em gestantes portadoras de pré-eclâmpsia
• Beneficiário: Mariana Romão Veiga
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 12/24697-8 - Envolvimento de inflamassomo e autofagia na fisiopatologia da pré-eclâmpsia
• Beneficiário: Maria Terezinha Serrão Peraçoli
• Modalidade de apoio: Auxílio à Pesquisa - Regular