| Full text | |
| Author(s): |
Romao-Veiga, Mariana
[1]
;
Matias, Mariana Leticia
[2]
;
Ribeiro, Vanessa Rocha
[2]
;
Nunes, Priscila Rezeck
[2]
;
Borges, Vera Therezinha M.
[2]
;
Peracoli, Jose Carlos
[2]
;
Peracoli, Maria Terezinha S.
[1]
Total Authors: 7
|
| Affiliation: | [1] Sao Paulo State Univ, UNESP, Inst Biosci, Dept Microbiol & Immunol, Botucatu, SP - Brazil
[2] Sao Paulo State Univ, UNESP, Botucatu Med Sch, Dept Gynaecol & Obstet, Botucatu, SP - Brazil
Total Affiliations: 2
|
| Document type: | Journal article |
| Source: | CYTOKINE; v. 105, p. 23-31, MAY 2018. |
| Web of Science Citations: | 6 |
| Abstract | |
Pre-eclampsia (PE) is a human pregnancy syndrome with abnormal activation of the innate immune response. The study evaluated the involvement of molecular structures called damage-associated molecular patterns (DAMPs), such as hyaluronan (HA) and heat shock proteins (Hsp) on NLRP1 and NLRP3 inflammasomes activation in peripheral blood monocytes. Twenty pre-eclamptic women, 20 normotensive pregnant women (NT) and 20 non-pregnant women (NP) were studied. Enzyme immunoassay was employed for the determination of HA, Hsp70 and High mobility group Box 1 (HMGB1) in plasma, as well as for the detection of Interleukin-1 beta (IL-1 beta), IL-18 and tumor necrosis factor alpha (TNF-alpha) in the supernatant of monocytes cultured with or without HA and Hsp70. The inflammasomes induction was evaluated by the quantification of mRNA for NLRP1, NLRP3, caspase-1, IL-1 beta, IL-18, HMGB1 and TNF-alpha by qPCR in monocyte culture. The results showed significantly higher plasma levels of HA, Hsp70 and HMGB1 in pre-eclamptic women than in NT and NP women. Monocytes from women with PE showed endogenous activation of NLRP1 and NLRP3 inflammasomes, and expressed high amounts of IL-1 beta, IL-18, HMGB1 and TNF-alpha. The stimulation of monocytes with HA increased the gene expression of NLRP1, NLRP3, caspase-1, TNF-alpha, HMGB1 and IL-18 and the production of IL-1 beta in preeclamptic women. Monocytes cultured with Hsp70 produced elevated levels of IL-1 beta and TNF-alpha through a mechanism independent of inflammasomes activation. These results suggest the participation of these DAMPs in the systemic inflammatory response that is characteristic of PE. (AU) | |
| FAPESP's process: | 13/00534-5 - The role of inflammasome in the innate and adaptive immunity in pregnant women with preeclampsia |
| Grantee: | Mariana Romão Veiga |
| Support Opportunities: | Scholarships in Brazil - Doctorate |
| FAPESP's process: | 12/24697-8 - Inflammasome and autophagy involvement in the pathophysiology of preeclampsia |
| Grantee: | Maria Terezinha Serrão Peraçoli |
| Support Opportunities: | Regular Research Grants |