Aerobic exercise inhibits obesity-induced respiratory phenotype

Autor(es):Mostrar menos -

Jonco Aquino-Junior, Jefferson Comin ^[1] ; MacKenzie, BreAnne ^[2] ; Almeida-Oliveira, Ana Roberta ^[1] ; Martins, Ana Carolina ; Oliveira-Junior, Manoel Carneiro ^{[1, 2]} ; Britto, Aurilea Aparecida ^[1] ; Arantes-Costa, Fernanda Magalhaes ^{[3, 4]} ; Damaceno-Rodrigues, Nilsa Regina ^[5] ; Caldini, Elia Garcia ^[5] ; Ligeiro de Oliveira, Ana Paula ^{[1, 2]} ; Guadagnini, Dioze ; Leiria, Luiz Osorio ; Ricardo, Djalma Rabelo ^[6] ; Abdalla Saad, Mario Jose ; Vieira, Rodolfo Paula ^{[2, 6, 7]}

Afiliação do(s) autor(es)

^[1] Nove de Julho Univ, Rua Vergueiro 235-249, BR-01504001 Sao Paulo, SP - Brazil

^[2] Brazilian Inst Teaching & Res Pulm & Exercise Imm, Rua Pedro Ernesto 240, BR-12245520 Sao Jose Dos Campos, SP - Brazil

^[3] Univ Sao Paulo, Sch Med, Lab Expt Therapeut LIM 20, Ave Doutor Arnaldo 455, BR-01246903 Sao Paulo, SP - Brazil

^[4] State Univ Campinas UNICAMP, Dept Internal Med, Rua Tessalia Vieira de Camargo 126, BR-13083887 Campinas, SP - Brazil

^[5] Univ Sao Paulo, Sch Med, Lab Cellular Biol LIM 59, Ave Doutor Arnaldo 455, BR-01246903 Sao Paulo, SP - Brazil

^[6] Sch Med Sci Sao Jose dos Campos Humanitas, Ave Brigadeiro Faria Lima 811, BR-12227000 Sao Jose Dos Campos, SP - Brazil

^[7] Univ Brasil, Rua Carolina Fonseca, 584 Itaquera, BR-08230030 Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: CYTOKINE; v. 104, p. 46-52, APR 2018.
• Citações Web of Science: 1

Resumo

Purpose: Obesity results in decreased lung function and increased inflammation. Moderate aerobic exercise (AE) reduced lung inflammation and remodeling in a variety of respiratory disease models. Therefore, this study investigated whether AE can attenuate a diet-induced obesity respiratory phenotype; including airway hyperresponsiveness (AHR), remodeling and inflammation.\& para;\& para;Methods: Sixty C57B1/6 male mice were distributed into four groups: control lean (CL), exercise lean (EL), obese (O) and obese exercise (OE) groups (2 sets of 7 and 8 mice per group; n = 15). A classical model of diet-induced obesity (DIO) over 12 weeks was used. AE was performed 60 min/day, 5 days/week for 5 weeks. Airway hyperresponsiveness (AHR), lung inflammation and remodeling, adipokines and cytokines in bronchoalveolar lavage (BAL) was determined.\& para;\& para;Results: A high fat diet over 18 weeks significantly increased body weight (p < .0001). Five weeks of AE significantly reduced both AHR and pulmonary inflammation. AHR in obese mice that exercised was reduced at the basal level (p < .05), vehicle (PBS) (p < .05), 6.25 MCh mg/mL (p < .05), 12.5 MCh mg/mL (p < .01), 25 MCh mg/mL (p < .01) and 50 MCh mg/mL (p < .05). Collagen (p < .001) and elastic (p < .001) fiber deposition in airway wall and also smooth muscle thickness (p < .001) were reduced. The number of neutrophils (p < .001), macrophages (p < .001) and lymphocytes (p < .01) were reduced in the peribronchial space as well as in the BAL: lymphocytes (p < .01), macrophages (p < .01), neutrophils (p < .001). AE reduced obesity markers leptin (p < .001), IGF-1 (p < .01) and VEGF (p < .001), while increased adiponectin (p < .01) in BAL. AE also reduced pro-inflammatory cytokines in the SAL: IL-1 beta (p < .001), IL-12p40 (p < .001), IL-13 (p < .01), IL-17 (p < .001, IL-23 (p < .05) and TNF-alpha (p < .05), and increased antiinflammatory cytokine IL-10 (p < .05).\& para;\& para;Conclusions: Aerobic exercise reduces high fat diet-induced obese lung phenotype (AHR, pulmonary remodeling and inflammation), involving anti-inflammatory cytokine IL-10 and adiponectin. (AU)

• Processo FAPESP: 14/14604-8 - Papel da sinalização purinérgica nos efeitos anti-inflamatórios do treinamento aeróbio em um modelo de asma induzida por ácaro de poeira doméstica
• Beneficiário: Manoel Carneiro de Oliveira Junior
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 14/07500-1 - Papel da sinalização SOCS-JAK-STAT nos efeitos antiinflamatórios do treinamento aeróbio em modelo experimental de asma
• Beneficiário: Ana Roberta Almeida Comin
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/15165-2 - Papel da sinalização purinérgica e da sinalização SOCS-JAK-STAT nos efeitos antiinflamatórios do treinamento aeróbio em modelos experimentais de asma e em indivíduos asmáticos
• Beneficiário: Rodolfo de Paula Vieira
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores