Chronic kidney disease induces inflammation by increasing Toll-like receptor-4, cytokine and cathelicidin expression in neutrophils and monocytes

Autor(es)

Grabulosa, Caren C. ^[1] ; Manfredi, Silvia R. ^[1] ; Canziani, Maria E. ^[1] ; Quinto, Beata M. R. ^[1] ; Barbosa, Rodrigo B. ^[2] ; Rebello, Jacqueline F. ^[2] ; Batista, Marcelo C. ^{[3, 4, 1]} ; Cendoroglo, Miguel ^{[3, 4, 1]} ; Dalboni, Maria A. ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo, Nephrol Div, Sao Paulo - Brazil

^[2] Univ Nove Julho, Sao Paulo - Brazil

^[3] Hosp Israelita Albert Einstein, Sao Paulo - Brazil

^[4] Tufts Univ New England Med Ctr, Boston, MA - USA

• Tipo de documento: Artigo Científico
• Fonte: Experimental Cell Research; v. 365, n. 2, p. 157-162, APR 15 2018.
• Citações Web of Science: 5

Resumo

TLR expression in neutrophils and monocytes is associated with increased cytokine synthesis, resulting in increased inflammation. However, the inflammatory pathway related to TLR and cathelicidin expression in these cells from CKD patients is unclear. To evaluate TLR4, cathelicidin, TNF-alpha, IL-6, IL-10 and MCP-1 expression in neutrophils and monocytes from HD and CKD patients. Blood samples were drawn from 47 CKD and 43 HD patients and 71 age and gender-matched healthy volunteers (CONT). TLR4 was analyzed using flow cytometry. Cathelicidin, TNF-alpha, IL-6, IL-10 and MCP-1 were analyzed via ELISA.TLR4 expression in neutrophils was higher in HD patients than in stage 3 and 4 CKD patients. In these cells, we observed a positive correlation between TLR4 and cathelicidin, TNF-alpha, IL-6, IL-10 and MCP-1 levels. In monocytes, TLR4 expression was significantly higher in CKD 3 and 4 groups than in the control and HD groups and positively and negatively correlated with IL-6 and MCP-1 and cathelicidin, respectively. TNF-alpha, IL-6 and MCP-1 serum levels were higher in HD and CKD patients than in control. Cathelicidin and IL-10 levels were only higher in HD patients. IL-6 serum levels were positively correlated with all cytokines, and cathelicidin was negatively correlated with MCP-1 (r = - 0.35; p < 0.01) and positively correlated with IL-10 (r = 0.37; p = 0.001). These results suggest that a uremic environment induces high TLR4, cathelicidin and cytokine expression and may increase inflammation. Thus, future studies should be conducted to evaluate whether TLR4 and cathelicidin should be targets for anti-inflammatory therapeutic strategies. (AU)

• Processo FAPESP: 11/51496-0 - Expressão dos receptores TLR-2 e TLR-4 em leucócitos de pacientes com doença renal crônica (DRC)
• Beneficiário: Maria Aparecida Dalboni
• Modalidade de apoio: Auxílio à Pesquisa - Regular