Combined treatment with melatonin and insulin improves glycemic control, white adipose tissue metabolism and reproductive axis of diabetic male rats

Autor(es)

de Oliveira, Ariclecio Cunha ^{[1, 2]} ; Andreotti, Sandra ^[1] ; Laurato Sertie, Rogerio Antonio ^[1] ; Campana, Amanda Baron ^[1] ; Gomes de Proenca, Andre Ricardo ^[1] ; Vasconcelos, Renata Prado ^[2] ; de Oliveira, Keciany Alves ^[2] ; Coelho-de-Souza, Andrelina Noronha ^[2] ; Donato-Junior, Jose ^[1] ; Lima, Fabio Bessa ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Av Prof Lineu Prestes 1524, Sao Paulo, SP - Brazil

^[2] Univ Estadual Ceara, Super Inst Biomed Sci, Av Silas Munguba 1700, Fortaleza, Ceara - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 199, p. 158-166, APR 15 2018.
• Citações Web of Science: 6

Resumo

Aims: Melatonin treatment has been reported to be capable of ameliorating metabolic diabetes-related abnormalities but also to cause hypogonadism in rats. We investigated whether the combined treatment with melatonin and insulin can improve insulin resistance and other metabolic disorders in rats with streptozotocininduced diabetes during neonatal period and the repercussion of this treatment on the hypothalamic-pituitarygonadal axis. Main methods: At the fourth week of age, diabetic animals started an 8-wk treatment with only melatonin (0.2 mg/kg body weight) added to drinking water at night or associated with insulin (NHP, 1.5U/100g/day) or only insulin. Animals were then euthanized, and the subcutaneous (SC), epididymal (EP), and retroperitoneal (RP) fat pads were excised, weighed and processed for adipocyte isolation for morphometric analysis as well as for measuring glucose uptake, oxidation, and incorporation of glucose into lipids. Hypothalamus was collected for gene expression and blood samples were collected for biochemical assays. Key findings: The treatment with melatonin plus insulin (MI) was capable of maintaining glycemic control. In epididymal (EP) and subcutaneous (SC) adipocytes, the melatonin plus insulin (MI) treatment group recovered the insulin responsiveness. In the hypothalamus, melatonin treatment alone promoted a significant reduction in kisspeptin-1, neurokinin B and androgen receptor mRNA levels, in relation to control group. Significance: Combined treatment with melatonin and insulin promoted a better glycemic control, improving insulin sensitivity in white adipose tissue (WAT). Indeed, melatonin treatment reduced hypothalamic genes related to reproductive function. (AU)

• Processo FAPESP: 10/00246-1 - Consequências do Diabetes Mellitus neonatal para o desenvolvimento puberal do tecido adiposo em ratos machos. 1. Efeitos sobre a adipogênese. 2. Efeito do tratamento com tiazolidinedionas e melatonina
• Beneficiário: Fabio Bessa Lima
• Modalidade de apoio: Auxílio à Pesquisa - Regular