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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal

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Autor(es):
de Macedo, Erenildo Ferreira [1] ; Ducatti Formaggio, Daniela Maria [1] ; Santos, Nivia Salles [1] ; Tada, Dayane Batista [1]
Número total de Autores: 4
Afiliação do(s) autor(es):
[1] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, Rua Talim 330, BR-12231280 Sao Jose Dos Campos, SP - Brazil
Número total de Afiliações: 1
Tipo de documento: Artigo Científico
Fonte: SENSORS; v. 17, n. 12 DEC 2017.
Citações Web of Science: 4
Resumo

Although several researchers had reported on methodologies for surface plasmon resonance (SPR) signal amplification based on the use of nanoparticles (NPs), the majority addressed the sandwich technique and low protein concentration. In this work, a different approach for SPR signal enhancement based on the use of gold NPs was evaluated. The method was used in the detection of two lectins, peanut agglutinin (PNA) and concanavalin A (ConA). Gold NPs were functionalized with antibodies anti-PNA and anti-ConA, and these NPs were used as protein scavengers in a solution. After being incubated with solutions of PNA or ConA, the gold NPs coupled with the collected lectins were injected on the sensor containing the immobilized antibodies. The signal amplification provided by this method was compared to the signal amplification provided by the direct coupling of PNA and ConA to gold NPs. Furthermore, both methods, direct coupling and gold NPs as protein scavengers, were compared to the direct detection of PNA and ConA in solution. Compared to the analysis of free protein, the direct coupling of PNA and ConA to gold NPs resulted in a signal amplification of 10-40-fold and a 13-fold decrease of the limit of detection (LOD), whereas the use of gold NPs as protein scavengers resulted in an SPR signal 40-50-times higher and an LOD 64-times lower. (AU)

Processo FAPESP: 11/23895-8 - Caracterização e controle de mecanismos de internalização celular de nanopartículas
Beneficiário:Dayane Batista Tada
Linha de fomento: Auxílio à Pesquisa - Apoio a Jovens Pesquisadores
Processo FAPESP: 17/01697-6 - Síntese e funcionalização de nanopartículas bimetálicas para aplicação na terapia fotodinâmica de câncer
Beneficiário:Dayane Batista Tada
Linha de fomento: Auxílio à Pesquisa - Regular