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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Gold Nanoparticles Used as Protein Scavengers Enhance Surface Plasmon Resonance Signal

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Author(s):
de Macedo, Erenildo Ferreira [1] ; Ducatti Formaggio, Daniela Maria [1] ; Santos, Nivia Salles [1] ; Tada, Dayane Batista [1]
Total Authors: 4
Affiliation:
[1] Univ Fed Sao Paulo, Inst Ciencia & Tecnol, Rua Talim 330, BR-12231280 Sao Jose Dos Campos, SP - Brazil
Total Affiliations: 1
Document type: Journal article
Source: SENSORS; v. 17, n. 12 DEC 2017.
Web of Science Citations: 4
Abstract

Although several researchers had reported on methodologies for surface plasmon resonance (SPR) signal amplification based on the use of nanoparticles (NPs), the majority addressed the sandwich technique and low protein concentration. In this work, a different approach for SPR signal enhancement based on the use of gold NPs was evaluated. The method was used in the detection of two lectins, peanut agglutinin (PNA) and concanavalin A (ConA). Gold NPs were functionalized with antibodies anti-PNA and anti-ConA, and these NPs were used as protein scavengers in a solution. After being incubated with solutions of PNA or ConA, the gold NPs coupled with the collected lectins were injected on the sensor containing the immobilized antibodies. The signal amplification provided by this method was compared to the signal amplification provided by the direct coupling of PNA and ConA to gold NPs. Furthermore, both methods, direct coupling and gold NPs as protein scavengers, were compared to the direct detection of PNA and ConA in solution. Compared to the analysis of free protein, the direct coupling of PNA and ConA to gold NPs resulted in a signal amplification of 10-40-fold and a 13-fold decrease of the limit of detection (LOD), whereas the use of gold NPs as protein scavengers resulted in an SPR signal 40-50-times higher and an LOD 64-times lower. (AU)

FAPESP's process: 11/23895-8 - Characterization and control of cellular internalization process of nanoparticles
Grantee:Dayane Batista Tada
Support Opportunities: Research Grants - Young Investigators Grants
FAPESP's process: 17/01697-6 - Synthesis and functionalization of bimetallic nanoparticles for application in photodynamic therapy of cancer
Grantee:Dayane Batista Tada
Support Opportunities: Regular Research Grants