Structural Insights into Protein-Protein Interactions Involved in Bacterial Cell Wall Biogenesis

Autor(es)

Laddomada, Federica ^{[1, 2, 3]} ; Miyachiro, Mayara M. ^[4] ; Dessen, Andrea ^{[1, 2, 3, 4]}

Afiliação do(s) autor(es)

^[1] CNRS, IBS, F-38044 Grenoble - France

^[2] Univ Grenoble Alpes, IBS, F-38044 Grenoble - France

^[3] Commissariat Energie Atom & Energies Alternat CEA, IBS, F-38044 Grenoble - France

^[4] Brazilian Natl Lab Biosci LNBio, CNPEM, BR-13083100 Sao Paulo - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: ANTIBIOTICS-BASEL; v. 5, n. 2 JUN 2016.
• Citações Web of Science: 10

Resumo

The bacterial cell wall is essential for survival, and proteins that participate in its biosynthesis have been the targets of antibiotic development efforts for decades. The biosynthesis of its main component, the peptidoglycan, involves the coordinated action of proteins that are involved in multi-member complexes which are essential for cell division (the divisome) and/or cell wall elongation (the elongasome), in the case of rod-shaped cells. Our knowledge regarding these interactions has greatly benefitted from the visualization of different aspects of the bacterial cell wall and its cytoskeleton by cryoelectron microscopy and tomography, as well as genetic and biochemical screens that have complemented information from high resolution crystal structures of protein complexes involved in divisome or elongasome formation. This review summarizes structural and functional aspects of protein complexes involved in the cytoplasmic and membrane-related steps of peptidoglycan biosynthesis, with a particular focus on protein-protein interactions whereby disruption could lead to the development of novel antibacterial strategies. (AU)

• Processo FAPESP: 11/52067-6 - Estruturação de complexos macromoleculares da parede bacteriana: biossíntese e virulência
• Beneficiário: Andrea Dessen de Souza e Silva
• Modalidade de apoio: Auxílio à Pesquisa - Programa SPEC
• Processo FAPESP: 13/02451-0 - Caracterização estrutural de componentes de um complexo essencial para a formação da parede bacteriana
• Beneficiário: Mayara Mayele Miyachiro
• Modalidade de apoio: Bolsas no Brasil - Doutorado Direto