In Endothelial Cells, the Activation or Stimulation of Soluble Guanylyl Cyclase Induces the Nitric Oxide Production by a Mechanism Dependent of Nitric Oxide Synthase Activation

Autor(es)

Martinelli, Ariane Migliato ^[1] ; dos Santos Rodrigues, Carla Nascimento ^[1] ; de Moraes, Thiago Francisco ^[1] ; Rodrigues, Gerson Jhonatan ^[1]

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Carlos, Dept Ciencias Fisiol, Sao Carlos, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES; v. 21, p. 38-45, 2018.
• Citações Web of Science: 1

Resumo

Purpose. In endothelial cells, investigate if the soluble guanylate cyclase (sGC) activation or stimulation is able to potentiate the relaxation in vessels. Methods. Aortic and coronary rings with and without endothelium were placed in a myograph and cumulative concentration-effect curves for DETA-NO or ataciguat were performed. Nitric oxide (NO) were measured by fluorescence or by selective electrode in human umbilical endothelial cells (HUVECs) in response to some treatments, including ataciguat, 8-Br-cGMP and A23187. Results. The presence of the endothelium potentiated the relaxation induced by DETANO in aortic and coronary rings. In addition, in aortic rings the endothelium potentiated the relaxation induced by ataciguat. In the presence of nitric oxide synthase (NOS) inhibitor, the endothelium effect was abolished to DETANO or ataciguat, in both vessels. Ataciguat, 8-Br-cGMP and A23187 were able to induce NO production in HUVECs cells. In the presence of NOS inhibitor, the NO production induced by ataciguat and 8-Br-cGMP was abolished. Conclusions. Our results suggest that in aortic and coronary rings the endothelium potentiates the relaxation induced by activation or stimulation of sGC through a mechanism dependent of NOS activation. (AU)

• Processo FAPESP: 14/01341-9 - Utilização dos complexos de rutênio cis-[Ru(H-dcbpy-)2(Cl)(NO)] e cis-[Ru(bpy)2(NO2-)(NO)](PF6)2 para diminuir a disfunção endotelial em ratos hipertensos renais.
• Beneficiário: Carla Nascimento dos Santos Rodrigues
• Modalidade de apoio: Bolsas no Brasil - Programa Capacitação - Treinamento Técnico
• Processo FAPESP: 12/24477-8 - Utilização de complexos de rutênio como estratégia farmacológica para reverter e/ou prevenir a disfunção endotelial
• Beneficiário: Gerson Jhonatan Rodrigues
• Modalidade de apoio: Auxílio à Pesquisa - Jovens Pesquisadores