Enhanced immunization techniques to obtain highly specific monoclonal antibodies

Autor(es):Mostrar menos -

de Almeida, Rodrigo ^[1] ; Nakamura, Cecilia Naomi ^[1] ; de Lima Fontes, Marina ^[1] ; Deffune, Elenice ^[2] ; Felisbino, Sergio Luis ^[3] ; Kaneno, Ramon ^[4] ; Favaro, Wagner Jose ^[5] ; Billis, Athanase ^[6] ; Cerri, Marcel Otavio ^[7] ; Fusco-Almeida, Ana Marisa ^[1] ; Mendes Giannini, Maria Jose ^[1] ; Moroz, Andrei ^{[1, 7, 2]}

Afiliação do(s) autor(es)

^[1] Univ Estadual Paulista, Fac Ciencias Farmaceut, Prote Ctr, Monoclonal Antibody Lab, UNESP, Araraquara - Brazil

^[2] Univ Estadual Paulista, Fac Med, Blood Transfus Ctr, Cell Engn Lab, UNESP, Botucatu, SP - Brazil

^[3] Univ Estadual Paulista, Inst Biociencias, Dept Morphol, Extracellular Matrix Lab, UNESP, Botucatu, SP - Brazil

^[4] Univ Estadual Paulista, Inst Biociencias, Dept Microbiol & Immunol, Tumor Immunol Lab, UNESP, Botucatu, SP - Brazil

^[5] Univ Campinas UNICAMP, Biol Inst, Dept Struct & Funct Biol, Immunotherapy Lab, Campinas, SP - Brazil

^[6] Univ Estadual Campinas, Sch Med, Dept Pathol, UNICAMP, Uropathol Lab, Campinas, SP - Brazil

^[7] Univ Estadual Paulista, Fac Ciencias Farmaceut, Dept Bioproc & Biotechnol, UNESP, Araraquara - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: mAbs; v. 10, n. 1, p. 46-54, 2018.
• Citações Web of Science: 2

Resumo

Despite fast advances in genomics and proteomics, monoclonal antibodies (mAbs) are still a valuable tool for areas such as the evolution of basic research in stem cells and cancer, for immunophenotyping cell populations, diagnosing and prognosis of diseases, and for immunotherapy. To summarize different subtractive immunization approaches successfully used for the production of highly specific antibodies, we identified scientific articles in NCBI PubMed using the following search terms: subtractive immunization, monoclonal antibody, tolerization, neonatal, high-zone tolerance, masking immunization. Patent records were also consulted. From the list of results, we included all available reports, from 1985 to present, that used any enhanced immunization technique to produce either polyclonal or monoclonal antibodies. Our examination yielded direct evidence that these enhanced immunization techniques are efficient in obtaining specific antibodies to rare epitopes, with different applications, such as to identify food contaminants or tumor cells. (AU)

• Processo FAPESP: 15/21884-0 - Produção de anticorpos monoclonais murinos reconhecedores de células tumorais prostáticas
• Beneficiário: Rodrigo de Almeida
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 14/23738-8 - Produção de anticorpos monoclonais murinos contra antígenos associados à metástase de células tumorais prostáticas por imunização subtrativa
• Beneficiário: Andrei Moroz
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 16/07047-0 - Eficiência da imunização subtrativa na obtenção de anticorpos específicos a células tumorais prostáticas
• Beneficiário: Cecília Naomi Nakamura
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica