Skin Biomarkers for Cystic Fibrosis: A Potential Non-Invasive Approach for Patient Screening

Autor(es):Mostrar menos -

Esteves, Cibele Zanardi ^[1] ; Dias, Leticia de Aguiar ^[1] ; Lima, Estela de Oliveira ^[1] ; de Oliveira, Diogo Noin ^[1] ; Odir Rodrigues Melo, Carlos Fernando ^[1] ; Delafiori, Jeany ^[1] ; Souza Gomez, Carla Cristina ^[2] ; Ribeiro, Jose Dirceu ^[2] ; Ribeiro, Antonio Fernando ^[2] ; Levy, Carlos Emilio ^[3] ; Catharino, Rodrigo Ramos ^[1]

Afiliação do(s) autor(es)

^[1] Univ Estadual Campinas, Innovare Biomarkers Lab, Med & Expt Surg Nucleus, Campinas, SP - Brazil

^[2] Univ Estadual Campinas, Pediat Dept, Campinas, SP - Brazil

^[3] Univ Estadual Campinas, Dept Clin Pathol, Campinas, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FRONTIERS IN PEDIATRICS; v. 5, JAN 10 2018.
• Citações Web of Science: 2

Resumo

Background: Cystic fibrosis (CF) is a disabling genetic disease with an increased prevalence in European heritage populations. Currently, the most used technique for collection of CF samples and diagnosis is provided through uncomfortable tests, with uncertain results, mostly based on chloride concentration in sweat. Since CF mutation induces many metabolic changes in patients, exploring these alterations might be an alternative to visualize potential biomarkers that could be used as interesting tools for further diagnostic upgrade, prioritizing simplicity, low cost, and quickness. Methods: This contribution describes an accurate strategy to provide potential biomarkers related to CF, which may be understood as a potential tool for new diagnostic approaches and/or for monitoring disease evolution. Therefore, the present proposal consists of using skin imprints on silica plates as a way of sample collection, followed by direct-infusion high-resolution mass spectrometry and multivariate data analysis, intending to identify metabolic changes in skin composition of CF patients. Results: Metabolomics analysis allowed identifying chemical markers that can be traced back to CF in patients' skin imprints, differently from control subjects. Seven chemical markers from several molecular classes were elected, represented by bile acids, a glutaric acid derivative, thyrotropin-releasing hormone, an inflammatory mediator, a phosphatidic acid, and diacylglycerol isomers, all reflecting metabolic disturbances that occur due to of CF. Conclusion: The comfortable method of sample collection combined with the identified set of biomarkers represent potential tools that open the range of possibilities to manage CF and follow the disease evolution. This exploratory approach points to new perspectives about the development of diagnostic assay using biomarkers and the management CF. (AU)

• Processo FAPESP: 15/06809-1 - Determinação de marcadores químicos em diferentes produtos bovinos por Espectrometria de Massas
• Beneficiário: Rodrigo Ramos Catharino
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/00302-0 - Shotgun lipidômico em giardíase, meningites e tuberculose: a busca de marcadores para diagnósticos, empregando novas e revolucionárias técnicas de espectrometria de massas
• Beneficiário: Cibele Zanardi Esteves
• Modalidade de apoio: Bolsas no Brasil - Doutorado
• Processo FAPESP: 11/50400-0 - Metabolismo energético, estado redox e funcionalidade mitocondrial na morte celular e em desordens cardiometabólicas e neurodegenerativas
• Beneficiário: Aníbal Eugênio Vercesi
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 16/17066-2 - Metabolômica e lipidômica do Zika vírus, do mosquito ao paciente
• Beneficiário: Carlos Fernando Odir Rodrigues Melo
• Modalidade de apoio: Bolsas no Brasil - Doutorado