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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Skin Biomarkers for Cystic Fibrosis: A Potential Non-Invasive Approach for Patient Screening

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Esteves, Cibele Zanardi [1] ; Dias, Leticia de Aguiar [1] ; Lima, Estela de Oliveira [1] ; de Oliveira, Diogo Noin [1] ; Odir Rodrigues Melo, Carlos Fernando [1] ; Delafiori, Jeany [1] ; Souza Gomez, Carla Cristina [2] ; Ribeiro, Jose Dirceu [2] ; Ribeiro, Antonio Fernando [2] ; Levy, Carlos Emilio [3] ; Catharino, Rodrigo Ramos [1]
Total Authors: 11
[1] Univ Estadual Campinas, Innovare Biomarkers Lab, Med & Expt Surg Nucleus, Campinas, SP - Brazil
[2] Univ Estadual Campinas, Pediat Dept, Campinas, SP - Brazil
[3] Univ Estadual Campinas, Dept Clin Pathol, Campinas, SP - Brazil
Total Affiliations: 3
Document type: Journal article
Source: FRONTIERS IN PEDIATRICS; v. 5, JAN 10 2018.
Web of Science Citations: 2

Background: Cystic fibrosis (CF) is a disabling genetic disease with an increased prevalence in European heritage populations. Currently, the most used technique for collection of CF samples and diagnosis is provided through uncomfortable tests, with uncertain results, mostly based on chloride concentration in sweat. Since CF mutation induces many metabolic changes in patients, exploring these alterations might be an alternative to visualize potential biomarkers that could be used as interesting tools for further diagnostic upgrade, prioritizing simplicity, low cost, and quickness. Methods: This contribution describes an accurate strategy to provide potential biomarkers related to CF, which may be understood as a potential tool for new diagnostic approaches and/or for monitoring disease evolution. Therefore, the present proposal consists of using skin imprints on silica plates as a way of sample collection, followed by direct-infusion high-resolution mass spectrometry and multivariate data analysis, intending to identify metabolic changes in skin composition of CF patients. Results: Metabolomics analysis allowed identifying chemical markers that can be traced back to CF in patients' skin imprints, differently from control subjects. Seven chemical markers from several molecular classes were elected, represented by bile acids, a glutaric acid derivative, thyrotropin-releasing hormone, an inflammatory mediator, a phosphatidic acid, and diacylglycerol isomers, all reflecting metabolic disturbances that occur due to of CF. Conclusion: The comfortable method of sample collection combined with the identified set of biomarkers represent potential tools that open the range of possibilities to manage CF and follow the disease evolution. This exploratory approach points to new perspectives about the development of diagnostic assay using biomarkers and the management CF. (AU)

FAPESP's process: 15/06809-1 - Determination of chemical markers in various bovine products using mass spectrometry
Grantee:Rodrigo Ramos Catharino
Support Opportunities: Regular Research Grants
FAPESP's process: 14/00302-0 - Lipidomics shotgun in giardiasis, meningitis and tuberculosis: the search of diagnostic markers, using revolutionary new techniques of mass spectrometry
Grantee:Cibele Zanardi Esteves
Support Opportunities: Scholarships in Brazil - Doctorate
FAPESP's process: 11/50400-0 - Mitochondrial energy metabolism, redox state and functionality in cell death and cardiometabolic and neurodegenerative disorders
Grantee:Aníbal Eugênio Vercesi
Support Opportunities: Research Projects - Thematic Grants
FAPESP's process: 16/17066-2 - Metabolomics and lipidomics the Zika virus, from mosquitoes to the patient
Grantee:Carlos Fernando Odir Rodrigues Melo
Support Opportunities: Scholarships in Brazil - Doctorate