Metabolomics and lipidomics the Zika virus, from mosquitoes to the patient

• Grant number: 16/17066-2
• Support Opportunities: Scholarships in Brazil - Doctorate
• Start date: October 01, 2017
• End date: August 03, 2018
• Field of knowledge: Health Sciences - Medicine - Pathological Anatomy and Clinical Pathology
• Principal Investigator: Rodrigo Ramos Catharino
• Grantee: Carlos Fernando Odir Rodrigues Melo
• Host Institution: Faculdade de Ciências Médicas (FCM). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
• Associated scholarship(s): 18/03321-6 - A comprehensive metabolomics and lipidomics overview of plasma from patients with Alzheimer's Disease for screening dementia-linked biomarkers, BE.EP.DR

Abstract

Zika virus (ZIKV) is an arbovirus that plays an important role in increasing birth microcephalic infants and in adults it has been associated with Guillain-Barré syndrome. Arboviruses have adapted to urban mosquitoes facilitating the occurrence of major viral infection epidemics like Dengue virus and Zika virus. In turn, these mosquito-borne diseases were previously neglected, major gaps exist in biomedical information as the infection mechanism in the mosquito, infection mechanism in man and laboratory diagnosis. In parallel, metabolomics and lipidomics are revolutionary strategies that help to determine important biomarkers in controlling the viral infection in adult form of vectors, man infection and in the diagnosis of infectious diseases. Given this global emergency for public health and the revolutionary potential of the new "omics", this project aims to study the most important currently arbovirus: the Zika virus (ZIKV), proposing the search for biomarkers to: 1) identify and understand the viral mechanism infection in the mosquito for better virus control in urban areas; 2) identify and understand the viral mechanism infection in the man 3) development of a diagnostic method for ZIKV across platforms "omics", being an important screening aid to the laboratory diagnostic techniques already exist.

Scientific publications

(15)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

ODIR RODRIGUES MELO, CARLOS FERNANDO; DELAFIORI, JEANY; DABAJA, MOHAMAD ZIAD; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; COLOMBO, TATIANA ELIAS; NOGUEIRA, MAURICIO LACERDA; PROENCA-MODENA, JOSE LUIZ; CATHARINO, RODRIGO RAMOS. The role of lipids in the inception, maintenance and complications of dengue virus infection. SCIENTIFIC REPORTS, v. 8, AUG 7 2018. (15/06809-1, 11/50400-0, 13/21719-3, 16/17066-2)

GUERREIRO, TATIANE MELINA; DE OLIVEIRA, DIOGO NOIN; ODIR RODRIGUES MELO, CARLOS FERNANDO; LIMA, ESTELA DE OLIVEIRA; CATHARINO, RODRIGO RAMOS. Migration from plastic packaging into meat. Food Research International, v. 109, p. 320-324, JUL 2018. (15/06809-1, 16/17066-2)

DE OLIVEIRA, DIOGO N.; CAMARGO, ANA C. B.; MELO, CARLOS F. O. R.; CATHARINO, RODRIGO R.. A fast semi-quantitative screening for cocoa content in chocolates using MALDI-MSI. Food Research International, v. 103, p. 8-11, JAN 2018. (14/00084-2, 14/21791-9, 11/50400-0, 15/06809-1, 16/17066-2)

GUERREIRO, TATIANE MELINA; GONCALVES, ROSELI FERNANDES; RODRIGUES MELO, CARLOS FERNANDO O.; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; VISINTIN, JOSE ANTONIO; DE ACHILLES, MARCOS ANTONIO; CATHARINO, RODRIGO RAMOS. A Metabolomic Overview of Follicular Fluid in Cows. FRONTIERS IN VETERINARY SCIENCE, v. 5, FEB 8 2018. (15/06809-1, 11/18085-7, 11/50400-0, 16/17066-2, 10/01077-9)

ODIR RODRIGUES MELO, CARLOS FERNANDO; NAVARRO, LUIZ CLAUDIO; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; LIMA, ESTELA DE OLIVEIRA; DELAFIORI, JEANY; DABAJA, MOHAMED ZIAD; RIBEIRO, MARTA DA SILVA; DE MENEZES, MAICO; MARTINS RODRIGUES, RAFAEL GUSTAVO; et al. A Machine Learning Application Based in Random Forest for Integrating Mass Spectrometry-Based Metabolomic Data: A Simple Screening Method for Patients With Zika Virus. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 6, APR 11 2018. (14/00302-0, 11/50400-0, 15/06809-1, 16/17066-2, 17/12646-3)

MARTINS, PAULA; MACHADO, DAISY; THEIZEN, THAIS HOLTZ; OLIVEIRA GUARNIERI, JOAO PAULO; BERNARDES, BRUNO GAIA; GOMIDE, GABRIEL PICCIRILLO; FINZI CORAT, MARCUS ALEXANDRE; ABBEHAUSEN, CAMILLA; PROENCA MODENA, JOSE LUIZ; ODIR RODRIGUES MELO, CARLOS FERNANDO; et al. Outer Membrane Vesicles from Neisseria Meningitidis (Proteossome) Used for Nanostructured Zika Virus Vaccine Production. SCIENTIFIC REPORTS, v. 8, MAY 29 2018. (16/17066-2, 17/12719-0, 14/14457-5)

DELAFIORI, JEANY; LIMA, ESTELA DE OLIVEIRA; DABAJA, MOHAMED ZIAD; DIAS-AUDIBERT, FLAVIA LUISA; DE OLIVEIRA, DIOGO NOIN; RODRIGUES MELO, CARLOS FERNANDO ODIR; MORISHITA, KAREN NODA; SALES, GEOVANA MANZAN; TASCA GOIS RUIZ, ANA LUCIA; DA SILVA, GISELE GOULART; et al. Molecular signatures associated with prostate cancer cell line (PC-3) exposure to inactivated Zika virus. SCIENTIFIC REPORTS, v. 9, OCT 25 2019. (13/07607-8, 16/17066-2)

DABAJA, MOHAMED ZIAD; LIMA, ESTELA DE OLIVEIRA; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; ODIR RODRIGUES MELO, CARLOS FERNANDO; MORISHITA, KAREN NODA; LANCELLOTTI, MARCELO; TASCA GOIS RUIZ, ANA LUCIA; GOULART, GISELE; DUARTE, DIEGO ANDREAZZI; et al. Metabolic alterations induced by attenuated Zika virus in glioblastoma cells. CELL AND BIOSCIENCE, v. 8, SEP 4 2018. (15/06809-1, 11/50400-0, 16/17066-2, 13/07607-8)

GODOY FALCO, LUIS FERNANDO; ODIR RODRIGUES MELO, CARLOS FERNANDO; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; CATHARINO, RODRIGO RAMOS. An LDI-MSI approach for targeted and untargeted differentiation and assessment of pharmaceutical formulations. Talanta, v. 197, p. 92-97, MAY 15 2019. (11/50400-0, 15/06809-1, 16/17066-2)

LIMA, ESTELA DE O.; GUERREIRO, TATIANE M.; MELO, CARLOS FERNANDO O. R.; DE OLIVEIRA, DIOGO N.; MACHADO, DAISY; LANCELLOTI, MARCELO; CATHARINO, RODRIGO R.. MALDI imaging detects endogenous digoxin in glioblastoma cells infected by Zika virusWould it be the oncolytic key?. Journal of Mass Spectrometry, v. 53, n. 3, p. 257-263, MAR 2018. (15/06809-1, 14/00302-0, 11/50400-0, 16/17066-2)

ODIR RODRIGUES MELO, CARLOS FERNANDO; BACHUR, LUIS FELIPE; DELAFIORI, JEANY; DABAJA, MOHAMED ZIAD; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; TARARAM, CIBELE APARECIDA; BUSSO-LOPES, ARIANE FIDELIS; MORETTI, MARIA LUIZA; CATHARINO, RODRIGO RAMOS. Does leukotriene F4 play a major role in the infection mechanism of Candida sp.?. Microbial Pathogenesis, v. 149, DEC 2020. (16/17066-2, 11/50400-0, 18/03321-6, 15/06809-1)

MELO, CARLOS FERNANDO O. R.; DELAFIORI, JEANY; DE OLIVEIRA, DIOGO N.; GUERREIRO, TATIANE M.; ESTEVES, CIBELE Z.; LIMA, ESTELA DE O.; PANDO-ROBLES, VICTORIA; CATHARINO, RODRIGO R.; NETWORK, ZIKA-UNICAMP. Serum Metabolic Alterations upon Zika Infection. FRONTIERS IN MICROBIOLOGY, v. 8, OCT 10 2017. (15/06809-1, 14/00302-0, 11/50400-0, 16/17066-2)

GUERREIRO, TATIANE MELINA; DE OLIVEIRA, DIOGO NOIN; ODIR RODRIGUES MELO, CARLOS FERNANDO; LIMA, ESTELA DE OLIVEIRA; RIBEIRO, MARTA DA SILVA; CATHARINO, RODRIGO RAMOS. Evaluating the effects of the adulterants in milk using direct-infusion high resolution mass spectrometry. Food Research International, v. 108, p. 498-504, JUN 2018. (15/06809-1, 16/17066-2)

ESTEVES, CIBELE ZANARDI; DIAS, LETICIA DE AGUIAR; LIMA, ESTELA DE OLIVEIRA; DE OLIVEIRA, DIOGO NOIN; ODIR RODRIGUES MELO, CARLOS FERNANDO; DELAFIORI, JEANY; SOUZA GOMEZ, CARLA CRISTINA; RIBEIRO, JOSE DIRCEU; RIBEIRO, ANTONIO FERNANDO; LEVY, CARLOS EMILIO; et al. Skin Biomarkers for Cystic Fibrosis: A Potential Non-Invasive Approach for Patient Screening. FRONTIERS IN PEDIATRICS, v. 5, JAN 10 2018. (15/06809-1, 14/00302-0, 11/50400-0, 16/17066-2)

DE OLIVEIRA, DIOGO N.; LIMA, ESTELA O.; MELO, CARLOS F. O. R.; DELAFIORI, JEANY; GUERREIRO, TATIANE M.; RODRIGUES, RAFAEL G. M.; MORISHITA, KAREN N.; SILVEIRA, CYNTHIA; MURARO, STEFANIE PRIMON; DE SOUZA, GABRIELA FABIANO; et al. Inflammation markers in the saliva of infants born from Zika-infected mothers: exploring potential mechanisms of microcephaly during fetal development. SCIENTIFIC REPORTS, v. 9, SEP 20 2019. (18/03321-6, 11/50400-0, 13/07607-8, 16/17066-2)

Academic Publications

(References retrieved automatically from State of São Paulo Research Institutions)

MELO, Carlos Fernando Odir Rodrigues. Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient. 2019. Doctoral Thesis - Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas Campinas, SP.