Metabolomis and lipidomic of both Zika and Dengue virus infectious processes; from mosquito to the patient

Author(s)

Carlos Fernando Odir Rodrigues Melo

• Document type: Doctoral Thesis
• Press: Campinas, SP.
• Institution: Universidade Estadual de Campinas (UNICAMP). Faculdade de Ciências Médicas
• Defense date: 2019-09-23

Examining board members

Rodrigo Ramos Catharino; Karina Cogo Müller; Daniel Fábio Kawano; Daisy Machado; Rejane Maria Tommasini Grotto

• Advisor: Rodrigo Ramos Catharino

Abstract

Zika virus (ZIKV) is an arbovirus that plays an important role in increasing births of microcephalic babies and in adults and has been associated with Guillain-Barré syndrome and it has been of major public health concern. Dengue virus (DENV) is endemic in Brazil and, like ZIKV, is characterized by causing self-limiting infections in the vast majority of patients, being of great importance in public health due to economic losses due to decreased productivity in affected populations and above all in the hemorrhagic form of the disease that can lead to death. These two arboviruses have adapted to urban mosquitoes facilitating the occurrence of severe epidemics of Dengue and Zika since the A. aegypti (main vector) presents with gonotrophic discordance, which makes it an excellent vector. In general, mosquito-borne diseases have a tradition of being neglected, and there are gaps in important biomedical information such as mosquito infection mechanism, viral infection mechanism in man, and laboratory diagnosis. In parallel, metabolomics and lipidomics are strategic and revolutionary study methodologies that aid in the determination of important biomarkers in infection control and the global emergence of public health and the revolutionary potential of the new "omics"; which allows us to study mechanisms of infection in the adult form of the vectors in humans and is very important in the diagnosis. Given in context on screen, this project aimed to study the two most important arboviruses currently in Brazil: the ZIKV, proposing a search for biomarkers to identify and understand the mechanism of infection in the mosquito (Goal 1) and human (Goal 2) for its better disease control and development of a diagnostic method for ZIKV through "omic" platforms (Goal 3). For DENV the proposed goal was to search for biomarkers for DENV alterations in patients presenting with hemorrhagic Dengue (Goal 4) (AU)

• FAPESP's process: 16/17066-2 - Metabolomics and lipidomics the Zika virus, from mosquitoes to the patient
• Grantee: Carlos Fernando Odir Rodrigues Melo
• Support Opportunities: Scholarships in Brazil - Doctorate