Determination of chemical markers in various bovine products using mass spectrometry

• Grant number: 15/06809-1
• Support Opportunities: Regular Research Grants
• Start date: October 01, 2015
• End date: September 30, 2017
• Field of knowledge: Physical Sciences and Mathematics - Chemistry - Organic Chemistry
• Principal Investigator: Rodrigo Ramos Catharino
• Grantee: Rodrigo Ramos Catharino
• Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil

Abstract

The need for faster analytical methods with high efficiency is what drives the research applied to food science. Metabolomics is an extremely versatile platform that integrates statistical and experimental data of high reliability from analytical results. By allying modern mass spectrometry techniques to this extremely fertile segment, it is possible to carry out improvements and development. Samples of several beef products, follicular fluid, red meat and milk will be directly analyzed, with minimal sample preparation through Mass Spectrometry Imaging (MSI). Within this scope, ion sources such as matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) will be widely exploited. Structural elucidation will be supported by fragmentation reactions and analysis by high resolution mass spectrometry with ionization by electrospray (ESI-Orbitrap). The compounds (markers) may be pre-selected (target analysis) or identified post-analysis (metabolic fingerprinting). (AU)

Scientific publications

(20)

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

GUERREIRO, TATIANE MELINA; DE OLIVEIRA, DIOGO NOIN; ODIR RODRIGUES MELO, CARLOS FERNANDO; LIMA, ESTELA DE OLIVEIRA; RIBEIRO, MARTA DA SILVA; CATHARINO, RODRIGO RAMOS. Evaluating the effects of the adulterants in milk using direct-infusion high resolution mass spectrometry. Food Research International, v. 108, p. 498-504, JUN 2018. (15/06809-1, 16/17066-2)

MELO, CARLOS FERNANDO O. R.; DELAFIORI, JEANY; DE OLIVEIRA, DIOGO N.; GUERREIRO, TATIANE M.; ESTEVES, CIBELE Z.; LIMA, ESTELA DE O.; PANDO-ROBLES, VICTORIA; CATHARINO, RODRIGO R.; NETWORK, ZIKA-UNICAMP. Serum Metabolic Alterations upon Zika Infection. FRONTIERS IN MICROBIOLOGY, v. 8, OCT 10 2017. (15/06809-1, 14/00302-0, 11/50400-0, 16/17066-2)

ODIR RODRIGUES MELO, CARLOS FERNANDO; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; GUERREIRO, TATIANE MELINA; ESTEVES, CIBELE ZANARDI; BECK, RAISSA MARQUES; PADILLA, MARINA AIELLO; MILANEZ, GUILHERME PAIER; ARNS, CLARICE WEIS; PROENCA-MODENA, JOSE LUIZ; et al. A Lipidomics Approach in the Characterization of Zika-Infected Mosquito Cells: Potential Targets for Breaking the Transmission Cycle. PLoS One, v. 11, n. 10, OCT 10 2016. (15/06809-1, 14/00302-0, 11/50400-0, 14/00084-2, 13/11343-6)

ESTEVES, CIBELE ZANARDI; DIAS, LETICIA DE AGUIAR; LIMA, ESTELA DE OLIVEIRA; DE OLIVEIRA, DIOGO NOIN; ODIR RODRIGUES MELO, CARLOS FERNANDO; DELAFIORI, JEANY; SOUZA GOMEZ, CARLA CRISTINA; RIBEIRO, JOSE DIRCEU; RIBEIRO, ANTONIO FERNANDO; LEVY, CARLOS EMILIO; et al. Skin Biomarkers for Cystic Fibrosis: A Potential Non-Invasive Approach for Patient Screening. FRONTIERS IN PEDIATRICS, v. 5, JAN 10 2018. (15/06809-1, 14/00302-0, 11/50400-0, 16/17066-2)

GODOY FALCO, LUIS FERNANDO; ODIR RODRIGUES MELO, CARLOS FERNANDO; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; CATHARINO, RODRIGO RAMOS. An LDI-MSI approach for targeted and untargeted differentiation and assessment of pharmaceutical formulations. Talanta, v. 197, p. 92-97, MAY 15 2019. (11/50400-0, 15/06809-1, 16/17066-2)

LIMA, ESTELA DE O.; GUERREIRO, TATIANE M.; MELO, CARLOS FERNANDO O. R.; DE OLIVEIRA, DIOGO N.; MACHADO, DAISY; LANCELLOTI, MARCELO; CATHARINO, RODRIGO R.. MALDI imaging detects endogenous digoxin in glioblastoma cells infected by Zika virusWould it be the oncolytic key?. Journal of Mass Spectrometry, v. 53, n. 3, p. 257-263, MAR 2018. (15/06809-1, 14/00302-0, 11/50400-0, 16/17066-2)

DABAJA, MOHAMED ZIAD; LIMA, ESTELA DE OLIVEIRA; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; ODIR RODRIGUES MELO, CARLOS FERNANDO; MORISHITA, KAREN NODA; LANCELLOTTI, MARCELO; TASCA GOIS RUIZ, ANA LUCIA; GOULART, GISELE; DUARTE, DIEGO ANDREAZZI; et al. Metabolic alterations induced by attenuated Zika virus in glioblastoma cells. CELL AND BIOSCIENCE, v. 8, SEP 4 2018. (15/06809-1, 11/50400-0, 16/17066-2, 13/07607-8)

ODIR RODRIGUES MELO, CARLOS FERNANDO; BACHUR, LUIS FELIPE; DELAFIORI, JEANY; DABAJA, MOHAMED ZIAD; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; TARARAM, CIBELE APARECIDA; BUSSO-LOPES, ARIANE FIDELIS; MORETTI, MARIA LUIZA; CATHARINO, RODRIGO RAMOS. Does leukotriene F4 play a major role in the infection mechanism of Candida sp.?. Microbial Pathogenesis, v. 149, DEC 2020. (16/17066-2, 11/50400-0, 18/03321-6, 15/06809-1)

ODIR RODRIGUES MELO, CARLOS FERNANDO; ESTEVES, CIBELE ZANARDI; DE OLIVEIRA, ROSIMEIRE NUNES; GUERREIRO, TATIANE MELINA; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; MINE, JULIO CESAR; ALLEGRETTI, SILMARA MARQUES; CATHARINO, RODRIGO RAMOS. Early developmental stages of Ascaris lumbricoides featured by high-resolution mass spectrometry. Parasitology Research, v. 115, n. 11, p. 8-pg., 2016-11-01. (14/00084-2, 14/00302-0, 11/50400-0, 15/06809-1)

VIVIAN, ADRIANA FU; AOYAGUI, CAROLINE TIEMI; DE OLIVEIRA, DIOGO NOIN; CATHARINO, RODRIGO RAMOS. Mass spectrometry for the characterization of brewing process. Food Research International, v. 89, p. 8-pg., 2016-11-01. (14/00084-2, 15/06809-1)

VIVIAN, ADRIANA FU; AOYAGUI, CAROLINE TIEMI; DE OLIVEIRA, DIOGO NOIN; CATHARINO, RODRIGO RAMOS. Mass spectrometry for the characterization of brewing process. Food Research International, v. 89, n. 1, p. 281-288, NOV 2016. (15/06809-1, 14/00084-2)

ODIR RODRIGUES MELO, CARLOS FERNANDO; ESTEVES, CIBELE ZANARDI; DE OLIVEIRA, ROSIMEIRE NUNES; GUERREIRO, TATIANE MELINA; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; MINE, JULIO CESAR; ALLEGRETTI, SILMARA MARQUES; CATHARINO, RODRIGO RAMOS. Early developmental stages of Ascaris lumbricoides featured by high-resolution mass spectrometry. Parasitology Research, v. 115, n. 11, p. 4107-4114, NOV 2016. (14/00084-2, 15/06809-1, 14/00302-0, 11/50400-0)

DE OLIVEIRA, DIOGO N.; CAMARGO, ANA C. B.; MELO, CARLOS F. O. R.; CATHARINO, RODRIGO R.. A fast semi-quantitative screening for cocoa content in chocolates using MALDI-MSI. Food Research International, v. 103, p. 8-11, JAN 2018. (14/00084-2, 14/21791-9, 11/50400-0, 15/06809-1, 16/17066-2)

GUERREIRO, TATIANE MELINA; GONCALVES, ROSELI FERNANDES; RODRIGUES MELO, CARLOS FERNANDO O.; DE OLIVEIRA, DIOGO NOIN; LIMA, ESTELA DE OLIVEIRA; VISINTIN, JOSE ANTONIO; DE ACHILLES, MARCOS ANTONIO; CATHARINO, RODRIGO RAMOS. A Metabolomic Overview of Follicular Fluid in Cows. FRONTIERS IN VETERINARY SCIENCE, v. 5, FEB 8 2018. (15/06809-1, 11/18085-7, 11/50400-0, 16/17066-2, 10/01077-9)

ODIR RODRIGUES MELO, CARLOS FERNANDO; NAVARRO, LUIZ CLAUDIO; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; LIMA, ESTELA DE OLIVEIRA; DELAFIORI, JEANY; DABAJA, MOHAMED ZIAD; RIBEIRO, MARTA DA SILVA; DE MENEZES, MAICO; MARTINS RODRIGUES, RAFAEL GUSTAVO; et al. A Machine Learning Application Based in Random Forest for Integrating Mass Spectrometry-Based Metabolomic Data: A Simple Screening Method for Patients With Zika Virus. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 6, APR 11 2018. (14/00302-0, 11/50400-0, 15/06809-1, 16/17066-2, 17/12646-3)

GUERREIRO, TATIANE MELINA; OZAWA, KUMI SHIOTA; LIMA, ESTELA DE OLIVEIRA; ODIR RODRIGUES MELO, CARLOS FERNANDO; DE OLIVEIRA, DIOGO NOIN; DO NASCIMENTO TRIANO, SIMONE PEREIRA; CATHARINO, RODRIGO RAMOS. New Approach of QuEChERS and GC-MS Triple-Quadrupole for the Determination of Ethyl Carbamate Content in Brazilian cachacas. FRONTIERS IN NUTRITION, v. 5, APR 6 2018. (11/50400-0, 15/06809-1)

DE MENEZES, MAICO; DE OLIVEIRA, DIOGO NOIN; CATHARINO, RODRIGO RAMOS. Capillary-induced Homogenization of Matrix in Paper: A Powerful Approach for the Quantification of Active Pharmaceutical Ingredients Using Mass Spectrometry Imaging. SCIENTIFIC REPORTS, v. 6, JUL 21 2016. (15/06809-1, 11/50400-0, 14/00084-2)

FERREIRA, MONICA SIQUEIRA; DE OLIVEIRA, DIOGO NOIN; MESQUITA, CAROLINE COSTA; DE LIMA BARBOSA, ANA PAULA; ANHE, GABRIEL FORATO; CATHARINO, RODRIGO RAMOS. MALDI-MSI: a fast and reliable method for direct melatonin quantification in biological fluids. JOURNAL OF ANALYTICAL SCIENCE AND TECHNOLOGY, v. 7, 2016. (14/00084-2, 11/50400-0, 15/06809-1)

ODIR RODRIGUES MELO, CARLOS FERNANDO; DELAFIORI, JEANY; DABAJA, MOHAMAD ZIAD; DE OLIVEIRA, DIOGO NOIN; GUERREIRO, TATIANE MELINA; COLOMBO, TATIANA ELIAS; NOGUEIRA, MAURICIO LACERDA; PROENCA-MODENA, JOSE LUIZ; CATHARINO, RODRIGO RAMOS. The role of lipids in the inception, maintenance and complications of dengue virus infection. SCIENTIFIC REPORTS, v. 8, AUG 7 2018. (15/06809-1, 11/50400-0, 13/21719-3, 16/17066-2)

GUERREIRO, TATIANE MELINA; DE OLIVEIRA, DIOGO NOIN; ODIR RODRIGUES MELO, CARLOS FERNANDO; LIMA, ESTELA DE OLIVEIRA; CATHARINO, RODRIGO RAMOS. Migration from plastic packaging into meat. Food Research International, v. 109, p. 320-324, JUL 2018. (15/06809-1, 16/17066-2)

Filed patent(s) as a result of this research project