Chronic activation profile of circulating CD8+T cells in Sezary syndrome

Autor(es):Mostrar menos -

Torrealba, Marina Passos ^[1] ; Manfrere, Kelly Cristina ^[1] ; Miyashiro, Denis R. ^[2] ; Lima, Josenilson F. ^[1] ; Oliveira, Luana de M. ^[1] ; Pereira, Natalli Z. ^[1] ; Cury-Martins, Jade ^[2] ; Pereira, Juliana ^[3] ; Duarte, Alberto J. S. ^[1] ; Sato, Maria N. ^[1] ; Sanches, Jose A. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Med Sch, Med Invest Lab LIM 56, Dept Dermatol, Trop Med Inst Sao Paulo, Sao Paulo - Brazil

^[2] Univ Sao Paulo, Med Sch, Dept Dermatol, Cutaneous Lymphoma Clin, Hosp Clin, Sao Paulo - Brazil

^[3] Univ Sao Paulo, Med Sch, Hosp Clin, Hematol Dept, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ONCOTARGET; v. 9, n. 3, p. 3497-3506, JAN 9 2018.
• Citações Web of Science: 2

Resumo

Sezary syndrome (SS) is a leukemic variant of cutaneous T cell lymphoma (CTCL), and the neoplastic CD4+ T cells of SS patients undergo intense clonal proliferation. Although Sezary cells have been studied extensively, studies on adaptive immunity regarding CD8+ T cells are scarce. This study aimed to investigate activation marker expression in CD8+ T cells according to the differentiation stages and IL-7/IL7Ra axis responses of patients with SS. Moreover, this study aimed to verify the soluble forms of CD38, sCD127 and IL-7 in serum. Although the SS patients of our cohort had reduced numbers of CD8+ T cells, they exhibited higher percentages of CD8+CD38+T cells, mainly effector/memory CD8+ T cells, than the control group. In contrast, down-regulated expression of the activation markers CD127/IL-7R and CD26 was found in the CD8+ T cells of SS patients. High serum levels of sCD38 and sCD127 and scarce serum levels of IL-7 were detected, emphasizing the immune activation status of SS patients. Moreover, CD8+ T cells from SS patients exhibited IL-7 unresponsiveness to STAT5 phosphorylation and Bcl-2 expression, and IL-7 priming partially restored IFN gamma production. Our findings showed a chronic activation profile of CD8+ T cells, as an attenuated cytotoxic profile and impaired IL-7 responsiveness was observed, suggesting chronic activation status of CD8+ T cells in SS patients. (AU)

• Processo FAPESP: 14/04865-9 - Ativação da resposta citotóxica mediada pelas células natural killer e TCD8+ polifuncionais por agonistas de receptores toll-like na micose fungóide e síndrome de Sézary
• Beneficiário: José Antonio Sanches Junior
• Modalidade de apoio: Auxílio à Pesquisa - Regular