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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

Friedelin in Maytenus ilicifolia Is Produced by Friedelin Synthase Isoforms

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Autor(es):
Alves, Thais B. [1] ; Souza-Moreira, Tatiana M. [2] ; Valentini, Sandro R. [2] ; Zanelli, Cleslei E. [2] ; Furlan, Maysa [1]
Número total de Autores: 5
Afiliação do(s) autor(es):
[1] Univ Estadual Paulista UNESP, Inst Quim, Rua Prof Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil
[2] Univ Estadual Paulista UNESP, Fac Ciencias Farmaceut, Rod Araraquara Jau Km 1, BR-14801903 Araraquara, SP - Brazil
Número total de Afiliações: 2
Tipo de documento: Artigo Científico
Fonte: Molecules; v. 23, n. 3 MAR 2018.
Citações Web of Science: 1
Resumo

Triterpenes are interesting compounds because they play an important role in cell homeostasis and a wide variety exhibiting defense functions is produced by plant secondary metabolism. Those same plant secondary metabolites also exhibit biological properties with promising therapeutic potential as anti-inflammatory and antitumor agents. Friedelin is a triterpene ketone with anti-inflammatory and gastroprotective activities and it is a precursor of relevant antitumor quinonemethides. Although many triterpene synthases have been described, only two friedelin synthases were characterized and there is no information about their genomic features and alleles. In the present work, we aimed to identify the gene and new isoforms of friedelin synthase in Maytenus ilicifolia leaves to be functionally characterized in Saccharomyces cerevisiae. The gene sequence analysis elucidated the exon/intron structure and confirmed the presence of single nucleotide polymorphisms with four non-synonymous mutations outside the active site of the enzyme. Therefore, two new isoforms were observed and the heterologous production of the enzymes in yeast showed similar production of friedelin. This first description of different alleles of the gene of friedelin synthase in M. ilicifolia can guide their validation as markers for friedelin-producer specimens. (AU)

Processo FAPESP: 14/03819-3 - Clonagem e caracterização funcional das oxidoesqualeno ciclases de Maytenus ilicifolia
Beneficiário:Cleslei Fernando Zanelli
Linha de fomento: Auxílio à Pesquisa - Regular
Processo FAPESP: 13/07600-3 - CIBFar - Centro de Inovação em Biodiversidade e Fármacos
Beneficiário:Glaucius Oliva
Linha de fomento: Auxílio à Pesquisa - Centros de Pesquisa, Inovação e Difusão - CEPIDs
Processo FAPESP: 11/10379-1 - Clonagem e caracterização de oxidoesqualeno ciclases de Maytenus ilicifolia Mart. ex Reissek
Beneficiário:Tatiana Maria de Souza Moreira
Linha de fomento: Bolsas no Brasil - Pós-Doutorado