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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Friedelin in Maytenus ilicifolia Is Produced by Friedelin Synthase Isoforms

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Author(s):
Alves, Thais B. [1] ; Souza-Moreira, Tatiana M. [2] ; Valentini, Sandro R. [2] ; Zanelli, Cleslei E. [2] ; Furlan, Maysa [1]
Total Authors: 5
Affiliation:
[1] Univ Estadual Paulista UNESP, Inst Quim, Rua Prof Francisco Degni 55, BR-14800060 Araraquara, SP - Brazil
[2] Univ Estadual Paulista UNESP, Fac Ciencias Farmaceut, Rod Araraquara Jau Km 1, BR-14801903 Araraquara, SP - Brazil
Total Affiliations: 2
Document type: Journal article
Source: Molecules; v. 23, n. 3 MAR 2018.
Web of Science Citations: 2
Abstract

Triterpenes are interesting compounds because they play an important role in cell homeostasis and a wide variety exhibiting defense functions is produced by plant secondary metabolism. Those same plant secondary metabolites also exhibit biological properties with promising therapeutic potential as anti-inflammatory and antitumor agents. Friedelin is a triterpene ketone with anti-inflammatory and gastroprotective activities and it is a precursor of relevant antitumor quinonemethides. Although many triterpene synthases have been described, only two friedelin synthases were characterized and there is no information about their genomic features and alleles. In the present work, we aimed to identify the gene and new isoforms of friedelin synthase in Maytenus ilicifolia leaves to be functionally characterized in Saccharomyces cerevisiae. The gene sequence analysis elucidated the exon/intron structure and confirmed the presence of single nucleotide polymorphisms with four non-synonymous mutations outside the active site of the enzyme. Therefore, two new isoforms were observed and the heterologous production of the enzymes in yeast showed similar production of friedelin. This first description of different alleles of the gene of friedelin synthase in M. ilicifolia can guide their validation as markers for friedelin-producer specimens. (AU)

FAPESP's process: 14/03819-3 - Cloning and functional characterization of oxidoesqualene cyclases of Maytenus ilicifolia
Grantee:Cleslei Fernando Zanelli
Support Opportunities: Regular Research Grants
FAPESP's process: 13/07600-3 - CIBFar - Center for Innovation in Biodiversity and Drug Discovery
Grantee:Glaucius Oliva
Support Opportunities: Research Grants - Research, Innovation and Dissemination Centers - RIDC
FAPESP's process: 11/10379-1 - CLONING AND CHARACTERIZATION OF OXIDOSQUALENE CYCLASES FROM Maytenus ilicifolia Mart. ex Reissek
Grantee:Tatiana Maria de Souza Moreira
Support Opportunities: Scholarships in Brazil - Post-Doctoral