Mesenchymal stromal cells modulate gut inflammation in experimental colitis

Autor(es)

de Aguiar, Cristhiane Favero ^[1] ; Castoldi, Angela ^[1] ; Andrade-Oliveira, Vinicius ^[1] ; Ignacio, Aline ^[1] ; da Cunha, Flavia Franco ^[2] ; Ferreira Felizardo, Raphael Jose ^[2] ; Bassi, Enio Jose ^[3] ; Saraiva Camara, Niels Olsen ^{[2, 1, 4, 5]} ; de Almeida, Danilo Candido ^{[2, 1, 4]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci 4, Dept Immunol, Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo, Nephrol Div, Dept Med, Sao Paulo - Brazil

^[3] Univ Fed Alagoas, Inst Biol Sci & Hlth, Alagoas - Brazil

^[4] Av Prof Lineu Prestes 1730 Lab 238 Cidade Univ, BR-05508000 Sao Paulo, SP - Brazil

^[5] Univ Sao Paulo, Sch Med, LIM 16, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: INFLAMMOPHARMACOLOGY; v. 26, n. 1, p. 251-260, FEB 2018.
• Citações Web of Science: 4

Resumo

Inflammatory bowel diseases (IBDs) affect millions of people worldwide and their frequencies in developed countries have increased since the twentieth century. In this context, there is an intensive search for therapies that modulate inflammation and provide tissue regeneration in IBDs. Recently, the immunomodulatory activity of adipose tissue-derived mesenchymal stromal cells (ADMSCs) has been demonstrated to play an important role on several immune cells in different conditions of inflammatory and autoimmune diseases. In this study, we explored the immunomodulatory potential of ADMSC in a classical model of DSS-induced colitis. First, we found that treatment of mice with ADMSC ameliorated the severity of DSS-induced colitis, reducing colitis pathological score and preventing colon shortening. Moreover, a prominent reduction of pro-inflammatory cytokines levels (i.e., IFN-gamma, TNF-alpha, IL-6 and MCP-1) was observed in the colon of animals treated with ADMSC. We also observed a significant reduction in the frequencies of macrophages (F4/80(+)CD11b(+)) and dendritic cells (CD11c(+)CD103(+)) in the intestinal lamina propria of ADMSC-treated mice. Finally, we detected the up-regulation of immunoregulatory-associated molecules in intestine of mice treated with ADMSCs (i.e., elevated arginase-1 and IL-10). Thus, this present study demonstrated that ADMSC modulates the overall gut inflammation (cell activation and recruitment) in experimental colitis, providing support to the further development of new strategies in the treatment of intestinal diseases. (AU)

• Processo FAPESP: 13/25327-2 - O papel do microambiente inflamatório na ativação e biologia das células-troncos mesenquimais
• Beneficiário: Danilo Candido de Almeida
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 12/02270-2 - Novos mecanismos celulares, moleculares e imunológicos das lesões renais agudas e crônicas: busca por novas estratégias terapêuticas
• Beneficiário: Niels Olsen Saraiva Câmara
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 12/16794-3 - Influência da microbiota intestinal na participação e no perfil das células NKT num modelo experimental de colite
• Beneficiário: Cristhiane Favero de Aguiar
• Modalidade de apoio: Bolsas no Brasil - Doutorado