Genetic diversity, anti-microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil

Autor(es)

Vilela, F. P. ^[1] ; Frazao, M. R. ^[2] ; Rodrigues, D. P. ^[3] ; Costa, R. G. ^[3] ; Casas, M. R. T. ^[4] ; Fernandes, S. A. ^[4] ; Falcao, J. P. ^[2] ; Campioni, F. ^[2]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Odontol Ribeirao Preto, Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, Dept Anal Clin Toxicol & Bromatol, Ribeirao Preto, SP - Brazil

^[3] Fiocruz MS, Fundacao Oswaldo Cruz, Rio De Janeiro, RJ - Brazil

^[4] Adolfo Lutz Inst, Ctr Bacteriol, Sao Paulo, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: ZOONOSES AND PUBLIC HEALTH; v. 65, n. 1, p. E34-E43, FEB 2018.
• Citações Web of Science: 2

Resumo

Salmonella Dublin is strongly adapted to cattle causing enteritis and/or systemic disease with high rates of mortality. However, it can be sporadically isolated from humans, usually causing serious disease, especially in patients with underlying chronic diseases. The aim of this study was to molecularly type S.Dublin strains isolated from humans and animals in Brazil to verify the diversity of these strains as well as to ascertain possible differences between strains isolated from humans and animals. Moreover, the presence of the capsular antigen Vi and the plasmid profile was characterized in addition to the anti-microbial resistance against 15 drugs. For this reason, 113 S.Dublin strains isolated between 1983 and 2016 from humans (83) and animals (30) in Brazil were typed by PFGE and MLVA. The presence of the capsular antigen Vi was verified by PCR, and the phenotypic expression of the capsular antigen was determined serologically. Also, a plasmid analysis for each strain was carried out. The strains studied were divided into 35 different PFGE types and 89 MLVA-types with a similarity of 80% and 17.5%, respectively. The plasmid sizes found ranged from 2 to >150kb and none of the strains studied presented the capsular antigen Vi. Resistance or intermediate resistance was found in 23 strains (20.3%) that were resistant to ampicillin, ciprofloxacin, chloramphenicol, imipenem, nalidixic acid, piperacillin, streptomycin and/or tetracycline. The majority of the S.Dublin strains studied and isolated over a 33-year period may descend from a common subtype that has been contaminating humans and animals in Brazil and able to cause invasive disease even in the absence of the capsular antigen. The higher diversity of resistance phenotypes in human isolates, as compared with animal strains, may be a reflection of the different anti-microbial treatments used to control S.Dublin infections in humans in Brazil. (AU)

• Processo FAPESP: 14/13029-0 - Caracterização molecular de linhagens de Campylobacter jejuni e Campylobacter coli de origens diversas
• Beneficiário: Juliana Pfrimer Falcão
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 15/10818-6 - Comparação fenotípica e genotípica de linhagens de Salmonella Dublin isoladas de humanos e animais no Brasil
• Beneficiário: Felipe Pinheiro Vilela
• Modalidade de apoio: Bolsas no Brasil - Iniciação Científica
• Processo FAPESP: 13/25191-3 - Análise fenotípica e sequenciamento do transcriptoma e genoma em linhagens de Salmonella Enteritidis isoladas no período pré e pós-pandemia no Brasil
• Beneficiário: Fábio Campioni
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado