Taurine supplementation induces long-term beneficial effects on glucose homeostasis in ob/ob mice

Autor(es)

Borck, Patricia Cristine ^[1] ; Vettorazzi, Jean Franciesco ^[1] ; Souto Branco, Renato Chaves ^[1] ; Batista, Thiago Martins ^[1] ; Santos-Silva, Junia Carolina ^[1] ; Nakanishi, Vanessa Yumi ^[1] ; Boschero, Antonio Carlos ^[1] ; Ribeiro, Rosane Aparecida ^[2] ; Carneiro, Everardo Magalhaes ^[1]

Afiliação do(s) autor(es)

^[1] Univ Campinas UNICAMP, Inst Biol, Lab Pancreas Endocrine & Metab, Obes & Comorbid Res Ctr, 255 Monteiro Lobato St, BR-13083862 Campinas, SP - Brazil

^[2] Univ Fed Rio de Janeiro, Campus UFRJ Macae, Macae, RJ - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Amino Acids; v. 50, n. 6, p. 765-774, JUN 2018.
• Citações Web of Science: 5

Resumo

The sulfur-containing amino acid, taurine (Tau), regulates glucose and lipid homeostasis under normal, pre- and diabetic conditions. Here, we aimed to verify whether Tau supplementation exerts its beneficial effects against obesity, hyperglycemia and alterations in islet functions, in leptin-deficient obese (ob/ob), over a long period of treatment. From weaning until 12 months of age, female ob/ob mice received, or not, 5% Tau in drinking water (obTau group). After this period, a reduction in hypertriglyceridemia and an improvement in glucose tolerance and insulin sensitivity were observed in obTau mice. In addition, the daily metabolic flexibility was restored in obTau mice. In the gastrocnemius muscle of obTau mice, the activation of AMP-activated protein kinase (AMPK) was increased, while total AMPK protein content was reduced. Finally, isolated islets from obTau mice expressed high amounts of pyruvate carboxylase (PC) protein and lower glucose-induced insulin secretion. Taking these evidences together Tau supplementation had long-term positive actions on glucose tolerance and insulin sensitivity, associated with a reduction in glucose-stimulated insulin secretion, in ob/ob mice. The improvement in insulin actions in obTau mice was due, at least in part, to increased activation of AMPK in skeletal muscle, while the increased content of the PC enzyme in pancreatic islets may help to preserve glucose responsiveness in obTau islets, possibly contributing to islet cell survive. (AU)

• Processo FAPESP: 14/01717-9 - Investigação dos efeitos insulinotrópicos, insulinomiméticos e endoteliais da taurina em células/tecidos submetidos à restrição in vitro de aminoácidos: uma abordagem integrada e multifocal
• Beneficiário: Everardo Magalhães Carneiro
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 15/12611-0 - Mecanismos moleculares envolvidos na disfunção e morte de células beta pancreáticas no Diabetes mellitus: estratégias para a inibição desses processos e para a recuperação da massa insular
• Beneficiário: Antonio Carlos Boschiero
• Modalidade de apoio: Auxílio à Pesquisa - Temático