Validation Cytotoxicity Assay for Lipophilic Substances

Autor(es):Mostrar menos -

Esteves-Pedro, Natalia Mencacci ^[1] ; Sugibayashi, Kenji ^[2] ; Ostrosky, Elissa A. ^[3] ; Ferrari, Marcio ^[3] ; Sufi, Bianca da Silva ^[4] ; Mathor, Monica Beatriz ^[4] ; Moreno, Paulo Roberto H. ^[1] ; Lourenco, Felipe R. ^[1] ; Consiglieri, Vladi Olga ^[1] ; Baby, Andre R. ^[1] ; Kaneko, Telma Mary ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Fac Ciencias Farmaceut, Dept Farm, POB CP 66083, BR-05315970 Sao Paulo - Brazil

^[2] Josai Univ, Fac Pharmaceut Sci, Sakado, Saitama - Japan

^[3] Univ Fed Rio Grande do Norte, Fac Farm, Natal, RN - Brazil

^[4] Inst Pesquisas Energet & Nucl, Sao Paulo - Brazil

• Tipo de documento: Artigo de Revisão
• Fonte: CURRENT TOPICS IN MEDICINAL CHEMISTRY; v. 18, n. 4, p. 275-286, 2018.
• Citações Web of Science: 1

Resumo

It is challenging to disperse lipophilic substances in a validated cytotoxicity assay, especially for compounds with log K-ow greater than or equal to 5 that may show false negative results. The purpose of this study was to explain the challenges in conducting a cytotoxicity validated test of lipophilic substances: Minthostachys setosa, Pimenta pseudocaryophyllus, and Drimysbrasiliensis essential oils. Additionally, we compared the equivalence of Neutral Red (NR) and 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl) -2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) in detecting cell viability. The Hydrophile-Lipophile Balance (HLB) technique was used to evaluate the dispersion of essential oils and cytotoxicity in accordance to the guidelines of the OECD / GD 129 validated cytotoxicity assay. We compared the equivalence of vital dyes by TOST equivalence test. According to the results, we demonstrated the possibility of using other ways to disperse the lipophilic substances. Based on the HLB theory, we selected polysorbate 20 as the best solubilizing agent of the essential oils studied in D10 culture medium (AU)

• Processo FAPESP: 10/03053-0 - Avaliação in vitro da toxicidade de óleos essenciais da flora latino-americana candidatos ao uso em cosméticos
• Beneficiário: Natália Mencacci Esteves-Pedro
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 10/20533-5 - Avaliação do óleo essencial da espécie nativa pimenta pseudocaryophyllus (Gomes) Landrum como promotor de permeação cutânea utilizando membrana natural e biológica artificial
• Beneficiário: Telma Mary Kaneko
• Modalidade de apoio: Auxílio à Pesquisa - Regular