High-fat diet affects gut nutrients transporters in hypo and hyperthyroid mice by PPAR-a independent mechanism

Autor(es)

Losacco, Mariana Cerqueira ^[1] ; Theodora de Almeida, Carolina Fernanda ^[1] ; Torelli Hijo, Andressa Harumi ^[1] ; Bargi-Souza, Paula ^[1] ; Gama, Patricia ^[2] ; Nunes, Maria Tereza ^[1] ; Goulart-Silva, Francemilson ^[1]

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Inst Biomed Sci, Dept Physiol & Biophys, Ave Prof Dr Lineu Prestes 1524, BR-05508000 Sao Paulo - Brazil

^[2] Univ Sao Paulo, Inst Biomed Sci, Dept Cell & Dev Biol, Sao Paulo - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Life Sciences; v. 202, p. 35-43, JUN 1 2018.
• Citações Web of Science: 5

Resumo

Aims: High fat diet consumes and thyroid hormones (THs) disorders may affect nutrients metabolism, but their impact on the absorptive epithelium, the first place of nutrients access, remains unknown. Our aim was to evaluate the intestinal morphology and nutrients transporters content in mice fed standard (LFD) or high fat (HFD) diets in hypo or hyperthyroidism-induced condition. Material and methods: C57BL/6 male mice fed LFD or HFD diets for 12 weeks, followed by saline, PTU (antithyroid drug) or T3 treatment up to 30 days. The mice were euthanized and proximal intestine was removed to study GLUT2, GLUT5, PEPT1, FAT-CD36, FATP4, NPC1L1 and NHE3 distribution by Western blotting. Since PPAR-a is activated by fatty acids, which is abundant in the HFD, we also evaluated whether PPAR-a affects nutrients transporters. Thus, mice were treated with fenofibrate, a PPAR-a agonist. Key findings: HFD decreased GLUT2, PEPT1, FAT-CD6 and NPC1L1, but increased NHE3, while GLUT5 and FATP4 remained unaltered. THs did not alter distribution of nutrients transporters neither in LFD nor in HFD groups, but they increased villi length and depth crypt in LFD and HFD, respectively. Fenofibrate did not affect content of nutrients transporters, excluding PPAR-a involvement on the HFD-induced changes. Significance: We assume that chronic HFD consumption reduced most of the nutrients transporters content in the small intestine of mice, which might limit the entrance of nutrients and gain weight. Since NHE3 promotes sodium absorption, and it was increased in HFD group, this finding could contribute to explain the hypertension observed in obesity. (AU)

• Processo FAPESP: 14/12871-9 - Repercussões da ingestão crônica de dieta hiperlipídica nos mecanismos de transporte de nutrientes dos enterócitos: avaliação da participação dos hormônios tireoidianos nesses processos
• Beneficiário: Francemilson Goulart da Silva
• Modalidade de apoio: Auxílio à Pesquisa - Regular