Recovery of the Paracoccidioides brasiliensis virulence after animal passage promotes changes in the antioxidant repertoire of the fungus

Autor(es)

Castilho, Daniele G. ^[1] ; Navarro, Marina V. ^[1] ; Chaves, Alison F. A. ^[1] ; Xander, Patricia ^[2] ; Batista, Wagner L. ^{[1, 2]}

Afiliação do(s) autor(es)

^[1] Univ Fed Sao Paulo UNIFESP, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo - Brazil

^[2] Univ Fed Sao Paulo UNIFESP, ICAQF, Dept Pharmaceut Sci, Campus Diadema, Rua Sao Nicolau 210, BR-09913030 Diadema - Brazil

• Tipo de documento: Artigo Científico
• Fonte: FEMS Yeast Research; v. 18, n. 2 MAR 2018.
• Citações Web of Science: 0

Resumo

Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis (PCM), a cause of disease in healthy and immunocompromised persons in Latin America. The infection begins after inhalation of the fungal propagules and their thermo-dimorphic shift to yeast form. The development of the disease depends on factors associated with the host immune response and the infectious agent's characteristics, especially virulence. The oxidative stress response is an important virulence attribute in several fungi. In this study, we assessed the enzymatic repertoire of responses to oxidative stress in the Pb18 isolate with different degrees of virulence. The virulence of attenuated Pb18 (aPb18) strain was recovered after several animal passages. Virulent strain (vPb18) showed an effective fungal oxidative stress response and several genes involved in response to oxidative stress were up-regulated in this isolate. These genes expressed the same profile when we recovered the phenotypic virulence in attenuated strain aPb18. Our study demonstrated that attenuated P. brasiliensis recovered their virulence after serial animal passages (vPb18), and this process positively modulated the fungus's antioxidant repertoire. (AU)

• Processo FAPESP: 14/13961-1 - Avaliação do papel da aspartil protease secretada (PbSAP) na virulência e no dimorfismo do fungo patogênico Paracoccidioides brasiliensis
• Beneficiário: Wagner Luiz Batista
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 17/04592-0 - Avaliação das proteínas S-nitrosiladas do fungo Paracoccidioides brasiliensis após estresse nitrosativo e identificação de potenciais vias de sinalização redox
• Beneficiário: Wagner Luiz Batista
• Modalidade de apoio: Auxílio à Pesquisa - Regular
• Processo FAPESP: 14/08987-1 - Estudo do papel da aspartil protease secretada (PbSAP) na virulência do fungo patogênico Paracoccidioides brasiliensis
• Beneficiário: Daniele Gonçalves Castilho
• Modalidade de apoio: Bolsas no Brasil - Doutorado