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(Reference retrieved automatically from Web of Science through information on FAPESP grant and its corresponding number as mentioned in the publication by the authors.)

Recovery of the Paracoccidioides brasiliensis virulence after animal passage promotes changes in the antioxidant repertoire of the fungus

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Castilho, Daniele G. [1] ; Navarro, Marina V. [1] ; Chaves, Alison F. A. [1] ; Xander, Patricia [2] ; Batista, Wagner L. [1, 2]
Total Authors: 5
[1] Univ Fed Sao Paulo UNIFESP, Dept Microbiol Immunol & Parasitol, BR-04023062 Sao Paulo - Brazil
[2] Univ Fed Sao Paulo UNIFESP, ICAQF, Dept Pharmaceut Sci, Campus Diadema, Rua Sao Nicolau 210, BR-09913030 Diadema - Brazil
Total Affiliations: 2
Document type: Journal article
Source: FEMS Yeast Research; v. 18, n. 2 MAR 2018.
Web of Science Citations: 0

Paracoccidioides brasiliensis is the agent of paracoccidioidomycosis (PCM), a cause of disease in healthy and immunocompromised persons in Latin America. The infection begins after inhalation of the fungal propagules and their thermo-dimorphic shift to yeast form. The development of the disease depends on factors associated with the host immune response and the infectious agent's characteristics, especially virulence. The oxidative stress response is an important virulence attribute in several fungi. In this study, we assessed the enzymatic repertoire of responses to oxidative stress in the Pb18 isolate with different degrees of virulence. The virulence of attenuated Pb18 (aPb18) strain was recovered after several animal passages. Virulent strain (vPb18) showed an effective fungal oxidative stress response and several genes involved in response to oxidative stress were up-regulated in this isolate. These genes expressed the same profile when we recovered the phenotypic virulence in attenuated strain aPb18. Our study demonstrated that attenuated P. brasiliensis recovered their virulence after serial animal passages (vPb18), and this process positively modulated the fungus's antioxidant repertoire. (AU)

FAPESP's process: 14/13961-1 - Role of secreted aspartyl protease (PbSAP) in virulence and dimorphism in the pathogenic fungus Paracoccidioides brasiliensis
Grantee:Wagner Luiz Batista
Support type: Regular Research Grants
FAPESP's process: 17/04592-0 - Evaluation of S-nitrosylated proteins of the Paracoccidioides brasiliensis after nitrosative stress and identification of potential redox signaling pathways
Grantee:Wagner Luiz Batista
Support type: Regular Research Grants
FAPESP's process: 14/08987-1 - Study of the role of secreted aspartyl protease (PbSAP) virulence of the pathogenic fungus Paracoccidioides brasiliensis
Grantee:Daniele Gonçalves Castilho
Support type: Scholarships in Brazil - Doctorate