Intense olfactory stimulation blocks seizures in an experimental model of epilepsy

Autor(es)

Delfino-Pereira, Polianna ^[1] ; Bertti-Dutra, Poliana ^{[2, 1]} ; de Lima Umeoka, Eduardo Henrique ^{[2, 1]} ; Cortes de Oliveira, Jose Antonio ^[2] ; Santos, Victor Rodrigues ^[2] ; Fernandes, Artur ^{[2, 3]} ; Marroni, Simone Saldanha ^{[2, 1]} ; Del Vecchio, Flavio ^[2] ; Garcia-Cairasco, Norberto ^{[2, 1]}

Afiliação do(s) autor(es)

^[1] Univ Sao Paulo, Hosp Clin, Ribeirao Preto Sch Med, Neurosci & Behav Sci Dept, Campus Univ S-N, 4 Andar, BR-14048900 Ribeirao Preto, SP - Brazil

^[2] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Physiol, Predio Cent, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Sch Med, Dept Genet, Ave Bandeirantes 3900, BR-14049900 Ribeirao Preto, SP - Brazil

• Tipo de documento: Artigo Científico
• Fonte: Epilepsy & Behavior; v. 79, p. 213-224, FEB 2018.
• Citações Web of Science: 0

Resumo

There are reports of patients whose epileptic seizures are prevented by means of olfactory stimulation. Similar findings were described in animal models of epilepsy, such as the electrical kindling of amygdala, where olfactory stimulation with toluene (TOL) suppressed seizures in most rats, even when the stimuli were 20% above the threshold to evoke seizures in already kindled animals. The Wistar Audiogenic Rat (WAR) strain is a model of tonic-clonic seizures induced by acute acoustic stimulation, although it also expresses limbic seizures when repeated acoustic stimulation occurs - a process known as audiogenic kindling (AK). The aim of this study was to evaluate whether or not the olfactory stimulation with TOL would interfere on the behavioral expression of brainstem(acute) and limbic (chronic) seizures in the WAR strain. For this, animals were exposed to TOL or saline (SAL) and subsequently exposed to acoustic stimulation in two conditions that generated: I) acute audiogenic seizures (only one acoustic stimulus, without previous seizure experience before of the odor test) and II) after AK (20 acoustic stimuli {[}2 daily] before of the protocol test). We observed a decrease in the seizure severity index of animals exposed only to TOL in both conditions, with TOL presented 20 s before the acoustic stimulation in both protocols. These findings were confirmed by behavioral sequential analysis (neuroethology), which clearly indicated an exacerbation of clusters of specific behaviors such as exploration and grooming (self-cleaning), as well as significant decrease in the expression of brainstem and limbic seizures in response to TOL. Thus, these data demonstrate that TOL, a strong olfactory stimulus, has anticonvulsant properties, detected by the decrease of acute and AK seizures in WARs. (C) 2017 Elsevier Inc. All rights reserved. (AU)

• Processo FAPESP: 12/18563-9 - A influência da estimulação olfatória no desenvolvimento de crises límbicas em ratos Wistar
• Beneficiário: Polianna Delfino Pereira
• Modalidade de apoio: Bolsas no Brasil - Mestrado
• Processo FAPESP: 12/18637-2 - A influência da estimulação olfatória no desenvolvimento de crises límbicas e mesencefálicas em ratos da cepa Wistar Audiogenic Rats - WAR
• Beneficiário: Poliana Bertti Dutra
• Modalidade de apoio: Bolsas no Brasil - Pós-Doutorado
• Processo FAPESP: 07/50261-4 - Neurociências, epilepsia e arte: caracterização interdisciplinar da cepa WAR e modelos de epilepsia correlacionados: as oficinas Da Vinci, divulgação científica e quebras de estigmas em epilepsia
• Beneficiário: Norberto Garcia Cairasco
• Modalidade de apoio: Auxílio à Pesquisa - Temático
• Processo FAPESP: 05/56447-7 - Pesquisa através de imagens por ressonância magnética de alto campo destinadas a estudos em humanos
• Beneficiário: João Pereira Leite
• Modalidade de apoio: Auxílio à Pesquisa - Programa CINAPCE - Temático