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(Referência obtida automaticamente do Web of Science, por meio da informação sobre o financiamento pela FAPESP e o número do processo correspondente, incluída na publicação pelos autores.)

The role of physical exercise on Sestrin1 and 2 accumulations in the skeletal muscle of mice

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Autor(es):
Crisol, Barbara M. [1] ; Lenhare, Luciene [1, 2] ; Gaspar, Rodrigo S. [1] ; Gaspar, Rafael C. [1] ; Munoz, Vitor R. [1] ; da Silva, Adelino S. R. [3] ; Cintra, Dennys E. [4] ; de Moura, Leandro P. [1, 5] ; Pauli, Jose R. [1, 5] ; Ropelle, Eduardo R. [1, 5, 2]
Número total de Autores: 10
Afiliação do(s) autor(es):
[1] Univ Estadual Campinas, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, Limeira, SP - Brazil
[2] Univ Estadual Campinas, Dept Internal Med, Fac Med Sci, UNICAMP, Campinas, SP - Brazil
[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Sch Phys Educ & Sport Ribeirao Preto, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP - Brazil
[4] Univ Estadual Campinas, Sch Appl Sci, Lab Nutr Genom LabGeN, Limeira, SP - Brazil
[5] Univ Estadual Campinas, Sch Appl Sci, CEPECE Ctr Res Sport Sci, UNICAMP, Limeira, SP - Brazil
Número total de Afiliações: 5
Tipo de documento: Artigo Científico
Fonte: Life Sciences; v. 194, p. 98-103, FEB 1 2018.
Citações Web of Science: 4
Resumo

Aims: Sestrins, a class of stress-related proteins, is involved in the control of aging-induced organic dysfunctions and metabolic control. However, the factors that modulate the levels of Sestrins are poorly studied. Here, we evaluated the effects of acute and chronic aerobic exercise on Sestrin 1 (Sesn1) and Sesn2 protein contents in the skeletal muscle of mice. Main methods: Male C57BL/6 J mice performed an acute or chronic (4 weeks) exercise protocols on a treadmill running at 60% of the peak workload. Then, the quadriceps muscle was removed and analyzed by Western blot. Bioinformatics analysis was also performed to evaluate Sesn1 and Sesn2 mRNA in the skeletal muscle and phenotypic pattern in a large panel of isogenic strains of BXD mice. Key findings: While acute aerobic exercise increased Sesn1 accumulation and induced a discrete augment of Sesn2 protein content and AMPK threonine phosphorylation, chronic exercise reduced the basal levels of Sesn1 and Sesn2 as well as of AMPK threonine phosphorylation in the quadriceps muscles of C57BL/6 J mice. In accordance with these experimental approaches, transcriptomic analysis revealed that Sesn1 and Sesn2 mRNA levels in the skeletal muscle were inversely correlated with the locomotor activity in several strains of BXD mice. (AU)

Processo FAPESP: 16/05499-1 - Efeitos da suplementação de nicotinamida Ribosoide sobre a UPR mitocondrial na musculatura esquelética e sobre a performance aeróbia de camundongos
Beneficiário:Barbara Moreira Crisol
Linha de fomento: Bolsas no Brasil - Mestrado
Processo FAPESP: 16/01089-3 - Avaliação da UPRmt e do desequilíbrio mitonuclear em resposta ao treinamento físico: Uma abordagem multi-orgânica.
Beneficiário:Eduardo Rochete Ropelle
Linha de fomento: Auxílio à Pesquisa - Regular