The role of physical exercise on Sestrin1 and 2 accumulations in the skeletal muscle of mice

Author(s)

Crisol, Barbara M. ^[1] ; Lenhare, Luciene ^{[1, 2]} ; Gaspar, Rodrigo S. ^[1] ; Gaspar, Rafael C. ^[1] ; Munoz, Vitor R. ^[1] ; da Silva, Adelino S. R. ^[3] ; Cintra, Dennys E. ^[4] ; de Moura, Leandro P. ^{[1, 5]} ; Pauli, Jose R. ^{[1, 5]} ; Ropelle, Eduardo R. ^{[1, 5, 2]}

Affiliation

^[1] Univ Estadual Campinas, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, Limeira, SP - Brazil

^[2] Univ Estadual Campinas, Dept Internal Med, Fac Med Sci, UNICAMP, Campinas, SP - Brazil

^[3] Univ Sao Paulo, Ribeirao Preto Med Sch, Sch Phys Educ & Sport Ribeirao Preto, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP - Brazil

^[4] Univ Estadual Campinas, Sch Appl Sci, Lab Nutr Genom LabGeN, Limeira, SP - Brazil

^[5] Univ Estadual Campinas, Sch Appl Sci, CEPECE Ctr Res Sport Sci, UNICAMP, Limeira, SP - Brazil

• Document type: Journal article
• Source: Life Sciences; v. 194, p. 98-103, FEB 1 2018.
• Web of Science Citations: 4

Abstract

Aims: Sestrins, a class of stress-related proteins, is involved in the control of aging-induced organic dysfunctions and metabolic control. However, the factors that modulate the levels of Sestrins are poorly studied. Here, we evaluated the effects of acute and chronic aerobic exercise on Sestrin 1 (Sesn1) and Sesn2 protein contents in the skeletal muscle of mice. Main methods: Male C57BL/6 J mice performed an acute or chronic (4 weeks) exercise protocols on a treadmill running at 60% of the peak workload. Then, the quadriceps muscle was removed and analyzed by Western blot. Bioinformatics analysis was also performed to evaluate Sesn1 and Sesn2 mRNA in the skeletal muscle and phenotypic pattern in a large panel of isogenic strains of BXD mice. Key findings: While acute aerobic exercise increased Sesn1 accumulation and induced a discrete augment of Sesn2 protein content and AMPK threonine phosphorylation, chronic exercise reduced the basal levels of Sesn1 and Sesn2 as well as of AMPK threonine phosphorylation in the quadriceps muscles of C57BL/6 J mice. In accordance with these experimental approaches, transcriptomic analysis revealed that Sesn1 and Sesn2 mRNA levels in the skeletal muscle were inversely correlated with the locomotor activity in several strains of BXD mice. (AU)

• FAPESP's process: 16/05499-1 - Effects of nicotinamide Ribosoide supplementation on mitochondrial UPR in skeletal muscle and the aerobic performance of mice
• Grantee: Barbara Moreira Crisol
• Support Opportunities: Scholarships in Brazil - Master
• FAPESP's process: 16/01089-3 - Evaluation of mtUPR and mito-nuclear imbalance in response to physical training: A multiorganic approach.
• Grantee: Eduardo Rochete Ropelle
• Support Opportunities: Regular Research Grants