Effects of nicotinamide Ribosoide supplementation on mitochondrial UPR in skeletal muscle and the aerobic performance of mice

• Grant number: 16/05499-1
• Support type: Scholarships in Brazil - Master
• Effective date (Start): March 01, 2017
• Effective date (End): February 28, 2018
• Field of knowledge: Biological Sciences - Physiology - Physiology of Effort
• Cooperation agreement: Coordination of Improvement of Higher Education Personnel (CAPES)
• Principal Investigator: Eduardo Rochete Ropelle
• Grantee: Barbara Moreira Crisol
• Home Institution: Faculdade de Ciências Aplicadas (FCA). Universidade Estadual de Campinas (UNICAMP). Limeira , SP, Brazil

Abstract

The molecule Nicotinamide Adenine Nucleotide (NAD+) is extremely important to control the energy homeostasis and as consequence of metabolic homeostasis. The increase in NAD+ levels is directly related to increase activity of Sirtuins (SIRTs), and these in turn are proteins known to trigger the process of Mitocondrial Unfolded Protein Response (UPRmt) and promote adaptation of skeletal muscles. In this line, studies show that supplementation with the NAD+ precursor , Nicotinamide Riboside (NR), it able to promoting improvement in the oxidative capacity of the mitochondria and mitochondrial biogenesis by triggering the UPRmt process. Studies also demonstrate that physcical training is able to promote adjustments in the organism as mitochondrial biogenesis, increase VO2max, changes in skeletal muscle fiber type, which when combined are responsible for the inscrease in oxidative metabolism and thereby increasing aerobic performance. Although it known about the effects of both physical training, as NR supplementation on improvement of mucle cells oxidative profile, the effects of the combination of physical training and NR supplementation have not been explored yet. In this way, the current Project aims to evaluate the combination of NR supplementation and physical training effects on the aerobic performance and triggering of UPRmt in skeletal muscle of mice. This project development will significantly contribute to understand the effects of aerobic training and NR supplementation in unleashing the UPRmt process and it is role on the skeletal muscle adaptations and aerobic performance. (AU)

Scientific publications

(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)

CRISOL, BARBARA M.; VEIGA, CAMILLA B.; BRAGA, RENATA R.; LENHARE, LUCIENE; BAPTISTA, IGOR L.; GASPAR, RAFAEL C.; MUNOZ, VITOR R.; CORDEIRO, ANDRE V.; DA SILVA, ADELINO S. R.; CINTRA, DENNYS E.; MOURA, LEANDRO P.; PAULI, JOSE R.; ROPELLE, EDUARDO R. NAD(+)precursor increases aerobic performance in mice. EUROPEAN JOURNAL OF NUTRITION, v. 59, n. 6, p. 2427-2437, SEP 2020. Web of Science Citations: 1.

CRISOL, BARBARA M.; VEIGA, CAMILLA B.; LENHARE, LUCIENE; BRAGA, RENATA R.; SILVA, VAGNER R. R.; DA SILVA, ADELINO S. R.; CINTRA, DENNYS E.; MOURA, LEANDRO P.; PAULI, JOSE R.; ROPELLE, EDUARDO R. Nicotinamide riboside induces a thermogenic response in lean mice. Life Sciences, v. 211, p. 1-7, OCT 15 2018. Web of Science Citations: 1.

CRISOL, BARBARA M.; LENHARE, LUCIENE; GASPAR, RODRIGO S.; GASPAR, RAFAEL C.; MUNOZ, VITOR R.; DA SILVA, ADELINO S. R.; CINTRA, DENNYS E.; DE MOURA, LEANDRO P.; PAULI, JOSE R.; ROPELLE, EDUARDO R. The role of physical exercise on Sestrin1 and 2 accumulations in the skeletal muscle of mice. Life Sciences, v. 194, p. 98-103, FEB 1 2018. Web of Science Citations: 4.